Complexity of translationally controlled transcription factor Sp3 isoform expression.

The Journal of Biological Chemistry
Alexandra SapetschnigGuntram Suske

Abstract

Sp3 is a ubiquitous transcription factor closely related to Sp1. Both proteins contain a highly conserved DNA-binding domain close to the C terminus and two glutamine-rich domains in the N-terminal moiety. Immunoblot analyses of Sp3 reveal a striking complex protein pattern of up to eight distinct species. This pattern is not observed in Sp3-deficient cell lines showing that all signals reflect Sp3 antigen. In this study, we have unraveled the complexity of Sp3 expression. We show that four isoforms of Sp3 that retain different parts of the N terminus are expressed in vivo. The four isoforms derive from alternative translational start sites at positions 1, 37, 856, and 907. An upstream open reading frame located at position -47 to -18 regulates expression of the two long isoforms. Unlike Sp1, none of the Sp3 isoforms is glycosylated. However, all four isoforms become SUMO-modified in vivo and in vitro specifically and exclusively at lysine residue 551. The transcriptional activity of the two long isoforms strongly depends on the promoter settings, whereas the small isoforms appear to be inactive. The transcriptional activity of all the Sp3 isoforms is regulated by SUMO modification. Our results demonstrate that Sp3 has many uni...Continue Reading

References

Jan 1, 1991·Annual Review of Biochemistry·J W Hershey
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S P Jackson, R Tjian
Aug 24, 1999·Nucleic Acids Research·S Philipsen, G Suske
Nov 26, 1999·Gene·G Suske
Jan 5, 2000·Molecular Cell·E Kowenz-Leutz, A Leutz
Jan 29, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T SuzukiM Horikoshi
Nov 14, 2000·Molecular and Cellular Biology·D R Morris, A P Geballe
Jan 2, 2001·The Journal of Biological Chemistry·M S RodriguezR T Hay
Mar 10, 2001·Molecular and Cellular Biology·M Kozak
Jul 27, 2001·Mechanisms of Development·H GöllnerG Suske
Nov 17, 2001·Molecular and Cellular Biology·R SchneiderM Wormington
Jan 29, 2002·Nucleic Acids Research·H BraunG Suske
Feb 28, 2002·Molecular Biology and Evolution·Kevin J Kolell, Douglas L Crawford
Oct 2, 2002·Molecular and Cellular Endocrinology·Peter Bouwman, Sjaak Philipsen
Oct 3, 2002·The EMBO Journal·Alexandra SapetschnigGuntram Suske
Nov 2, 2002·Molecular Biology and Evolution·Marjorie F Oleksiak, Douglas L Crawford
Nov 15, 2002·Journal of Cell Science·Stephan LangeElisabeth Ehler
Apr 22, 2003·Genes & Development·Cornelis F CalkhovenAchim Leutz
Jun 26, 2003·Nucleic Acids Research·Michael Zuker

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Citations

Jul 1, 2005·Molecular Biology of the Cell·Shihua HeJames R Davie
Jun 2, 2006·Molecular and Cellular Biology·Zihua GongRainer Renkawitz
Oct 10, 2007·Molecular and Cellular Biology·Pieter Fokko van LooAdriana C Gittenberger-de Groot
Aug 5, 2010·American Journal of Physiology. Endocrinology and Metabolism·Stephanie L Pierce, Sarah K England
Jul 1, 2005·Breast Cancer Research : BCR·Charletha V Irvin-Wilson, Gautam Chaudhuri
Jan 24, 2014·Molecular Biology of the Cell·Véronique N LafleurDarren E Richard
Oct 7, 2006·The Journal of Biological Chemistry·Xianrong MaoSteven W Barger
Jun 8, 2011·Molecular Pain·Catherine ChuMark A Schumacher
Sep 25, 2012·Biochemical and Biophysical Research Communications·Jingjing LiuJianlin Ren
Jul 30, 2011·Biochemical and Biophysical Research Communications·Vittoria InfantinoVito Iacobazzi
Sep 3, 2010·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Lin Li, James R Davie
Jul 1, 2008·The International Journal of Biochemistry & Cell Biology·Ludwig SchwarzmayrHans Rotheneder
Jan 12, 2008·Advances in Enzyme Regulation·James R DavieXuemei Wang
Apr 9, 2005·Journal of Neurochemistry·Jun Wang, Michael J Bannon
Sep 13, 2008·The FEBS Journal·Etienne RicherMathieu F M Cellier
Jun 1, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Tomohiro GotoNatsuo Yasui
Aug 2, 2007·Journal of Cellular Biochemistry·Shihua HeJames R Davie
Jul 8, 2008·European Journal of Immunology·Francisco Mora-LópezAntonio Campos-Caro
Jun 23, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Imme KrügerGuntram Suske
Jun 30, 2006·Biochimica Et Biophysica Acta·Xue ZhangRobert B Diasio
May 10, 2006·Biochemical and Biophysical Research Communications·Aida Rivera-GinesJane L Ko
Jul 19, 2005·Biochimica Et Biophysica Acta·Alicia TapiasVéronique Noé
Feb 11, 2010·The Biochemical Journal·Tracey E SwinglerIan M Clark

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