Component Interaction of ESCRT Complexes Is Essential for Endocytosis-Dependent Growth, Reproduction, DON Production and Full Virulence in Fusarium graminearum

Frontiers in Microbiology
Qiurong XieJie Zhou

Abstract

Multivesicular bodies (MVBs) are critical intermediates in the trafficking of ubiquitinated endocytosed surface proteins to the lysosome/vacuole for destruction. Recognizing and packaging ubiquitin modified cargoes to the MVB pathway require ESCRT (Endosomal sorting complexes required for transport) machinery, which consists of four core subcomplexes, ESCRT-0, ESCRT-I, ESCRT-II, and ESCRT-III. Fusarium graminearum is an important plant pathogen that causes head blight of major cereal crops. Our previous results showed that ESCRT-0 is essential for fungal development and pathogenicity in Fusarium graminearum. We then, in this study, systemically studied the protein-protein interactions within F. graminearum ESCRT-I, -II or -III complex, as well as between ESCRT-0 and ESCRT-I, ESCRT-I and ESCRT-II, and ESCRT-II and ESCRT-III complexes and found that loss of any ESCRT component resulted in abnormal function in endocytosis. In addition, ESCRT deletion mutants displayed severe defects in growth, deoxynivalenol (DON) production, virulence, sexual, and asexual reproduction. Importantly genetic complementation with corresponding ESCRT genes fully rescued all these defective phenotypes, indicating the essential role of ESCRT machinery i...Continue Reading

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Citations

Apr 16, 2020·Frontiers in Plant Science·Monica De CaroliGabriella Piro
Apr 15, 2020·Infection and Immunity·Yoon-Dong ParkPeter R Williamson
Jan 17, 2021·Applied and Environmental Microbiology·Chenyu WangHansong Dong

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Methods Mentioned

BETA
two-hybrid
PCR
phosphotransferase
gene knockout
environmental stresses
ELISA
fluorescence microscopy

Software Mentioned

ClonExpress

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