Composite system based on chitosan and room-temperature ionic liquid: direct electrochemistry and electrocatalysis of hemoglobin

Biomacromolecules
Xianbo LuJinghong Li

Abstract

A novel polymer/room-temperature ionic liquid (RTIL) composite material based on chitosan (Chi) and 1-butyl-3-methyl-imidazolium tetrafluoroborate (BMIM.BF(4)) was explored. The composite system can be readily used as an immobilization matrix to entrap proteins and enzymes. Hemoglobin (Hb) was chosen as a model protein to investigate the composite system. A pair of well-defined quasireversible redox peaks of hemoglobin were obtained at the Chi-BMIM.BF(4)-Hb composite-film-modified glassy carbon (GC) electrode by direct electron transfer between the protein and the GC electrode. Dramatically enhanced biocatalytic activity was exemplified at the Chi-BMIM.BF(4)-Hb/GC electrode by the reduction of oxygen and trichloroacetic acid. Thermogravimetric analysis (TGA) suggests that the Chi-BMIM.BF(4)-Hb composite has higher thermal stability than Chi-Hb itself. The Chi-BMIM.BF(4)-Hb film was also characterized by UV-visible spectra, indicating excellent stability in solution and good biocompatibility for protein. The unique composite material based on polymer and ionic liquid can find wide potential applications in direct electrochemistry, biosensors, and biocatalysis.

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Citations

Jul 20, 2010·Annual Review of Analytical Chemistry·Renee J Soukup-HeinDaniel W Armstrong
Dec 6, 2012·Biosensors & Bioelectronics·Rui FengQin Wei
Jan 7, 2014·Biosensors & Bioelectronics·Mehdi BaghayeriMoslem Mansour Lakouraj
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Dec 17, 2009·Analytical Biochemistry·Zhibin MaiXiaoyong Zou
Oct 3, 2008·Biosensors & Bioelectronics·Zong DaiXiaoyong Zou
Dec 18, 2007·Biosensors & Bioelectronics·Xianbo LuJinghong Li

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