PMID: 9171425Jun 1, 1997Paper

Composition and primary structure of the F1F0 ATP synthase from the obligately anaerobic bacterium Clostridium thermoaceticum

Journal of Bacteriology
A Das, L G Ljungdahl

Abstract

The subunit composition and primary structure of the proton-translocating F1F0 ATP synthase have been determined in Clostridium thermoaceticum. The isolated enzyme has a subunit composition identical to that of the F1F0 ATP synthase purified from Clostridium thermoautotrophicum (A. Das, D. M. Ivey, and L. G. Ljungdahl, J. Bacteriol. 179:1714-1720, 1997), both having six different polypeptides. The molecular masses of the six subunits were 60, 50, 32, 17, 19, and 8 kDa, and they were identified as alpha, beta, gamma, delta, epsilon, and c, respectively, based on their reactivity with antibodies against the F1 ATPase purified from C. thermoautotrophicum and by comparing their N-terminal amino acid sequences with that deduced from the cloned genes of the C. thermoaceticum atp operon. The subunits a and b found in many bacterial ATP synthases could not be detected either in the purified ATP synthase or crude membranes of C. thermoaceticum. The C. thermoaceticum atp operon contained nine genes arranged in the order atpI (i), atpB (a), atpE (c), atpF (b), atpH (delta), atpA (alpha), atpG (gamma), atpD (beta), and atpC (epsilon). The deduced protein sequences of the C. thermoaceticum ATP synthase subunits were comparable with those of...Continue Reading

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Citations

Jul 18, 2008·Environmental Microbiology·Elizabeth PierceStephen W Ragsdale
Oct 27, 2017·FEMS Microbiology Letters·Alexander Mayer, Dirk Weuster-Botz
Jun 8, 2011·Metallomics : Integrated Biometal Science·Güneş BenderStephen W Ragsdale
Jul 21, 2000·DNA Sequence : the Journal of DNA Sequencing and Mapping·T ExternbrinkP Dürre
Sep 2, 2003·Journal of Bacteriology·Amaresh Das, Lars G Ljungdahl
Nov 7, 2003·Applied and Environmental Microbiology·Volker Müller
Aug 12, 2000·Journal of Bacteriology·B J KoebmannP R Jensen
Sep 8, 1999·Microbiology and Molecular Biology Reviews : MMBR·G SchäferV Müller
Jul 14, 2004·Research in Microbiology·Harold L Drake, Steven L Daniel
Jan 6, 2005·Research in Microbiology·Harold L Drake, Steven L Daniel
Jul 1, 2021·Environmental Microbiology·Florian P RosenbaumMarie Charlotte Schoelmerich
Sep 5, 2021·Extremophiles : Life Under Extreme Conditions·Florian P Rosenbaum, Volker Müller

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