Composition and significance of detergent resistant membranes in mouse spermatozoa

Journal of Cellular Physiology
Brett NixonR John Aitken

Abstract

Mammalian spermatozoa acquire the ability to fertilize an oocyte as they ascend the female reproductive tract. This process is characterized by a complex cascade of biophysical and biochemical changes collectively know as "capacitation." The attainment of a capacitated state is accompanied by a dramatic reorganization of the surface architecture to render spermatozoa competent to recognize the oocyte and initiate fertilization. Emerging evidence indicates that this process is facilitated by molecular chaperone-mediated assembly of a multimeric receptor complex on the sperm surface. However, the mechanisms responsible for gathering key recognition molecules within this putative complex have yet to be defined. In this study, we provide the first evidence that chaperones partition into detergent resistant membrane fractions (DRMs) within capacitated mouse spermatozoa and co-localize in membrane microdomains enriched with the lipid raft marker, G(M1) ganglioside. During capacitation, these microdomains coalesce within the apical region of the sperm head, a location compatible with a role in sperm-zona pellucida interaction. Significantly, DRMs isolated from spermatozoa possessed the ability to selectively bind to the zona pellucida...Continue Reading

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Citations

Sep 2, 2011·The Journal of Biological Chemistry·Matthew D DunBrett Nixon
Sep 15, 2012·The Journal of Biological Chemistry·Andrew T ReidBrett Nixon
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Sep 30, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Graham D JohnsonStephen A Krawetz
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Nov 20, 2012·Proteomics·Mark A BakerR John Aitken
Jul 28, 2011·Journal of Cellular Physiology·Brett NixonR John Aitken
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