Composition of fibrin glues significantly influences axial vascularization and degradation in isolation chamber model

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
Andreas ArkudasUlrich Kneser

Abstract

In this study, different fibrin sealants with varying concentrations of the fibrin components were evaluated in terms of matrix degradation and vascularization in the arteriovenous loop (AVL) model of the rat. An AVL was placed in a Teflon isolation chamber filled with 500 μl fibrin gel. The matrix was composed of commercially available fibrin gels, namely Beriplast (Behring GmbH, Marburg, Germany) (group A), Evicel (Omrix Biopharmaceuticals S.A., Somerville, New Jersey, USA) (group B), Tisseel VH S/D (Baxter, Vienna, Austria) with a thrombin concentration of 4 IU/ml and a fibrinogen concentration of 80 mg/ml [Tisseel S F80 (Baxter), group C] and with an fibrinogen concentration of 20 mg/ml [Tisseel S F20 (Baxter), group D]. After 2 and 4 weeks, five constructs per group and time point were investigated using micro-computed tomography, and histological and morphometrical analysis techniques. The aprotinin, factor XIII and thrombin concentration did not affect the degree of clot degradation. An inverse relationship was found between fibrin matrix degradation and sprouting of blood vessels. By reducing the fibrinogen concentration in group D, a significantly decreased construct weight and an increased generation of vascularized c...Continue Reading

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Citations

Nov 30, 2012·Tissue Engineering. Part C, Methods·Andreas ArkudasUlrich Kneser
Sep 28, 2016·Plastic and Reconstructive Surgery·Nico LeibigVolker J Schmidt
Feb 16, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Volker J SchmidtUlrich Kneser

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