Composition of human zona pellucida as revealed by SDS-PAGE after silver staining

Molecular Reproduction and Development
S BercegeayP Barrière

Abstract

The macromolecular composition of zona pellucida (ZP) isolated from human oocytes and embryos was characterized by one- and two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (1-D- and 2-D-SDS-PAGE) under reducing conditions after silver staining. ZP specimens obtained after in vitro fertilization were removed from pools of heavily fragmented embryos and inseminated oocytes that failed to fertilize. For unfertilized oocytes, two major bands with an apparent molecular weight of, respectively, 96 and 76-54 kDa were observed after 1-D-SDS-PAGE and silver staining. When ZP were isolated from fragmented embryos, the electrophoretic pattern showed a marked attenuation of the 96-kDa band. Silver-stained 2-D-SDS-PAGE analysis of ZP components from unfertilized oocytes revealed the presence of four protein trains: ZP1 (Mr = 92-80 kDa, pl = 4.9-5.9), ZP2 (Mr = 66-58 kDa, pl = 5.0-6.0), ZP3H (high) (Mr = 72-58 kDa, pl = 3.5-5.1), and ZP3L (low) (Mr = 62-54 kDa, pl = 3.5-5.1). The human ZP3 family (ZP3H and ZP3L) showed marked heterogeneity. Fertilization-associated changes were apparent in the electrophoretic pattern. ZP1 (Mr = 92-86 kDa, pl = 5.0-5.8) displayed a dramatic decrease in intensity, and a new component ...Continue Reading

References

Jan 1, 1991·International Review of Cytology·K S Sidhu, S S Guraya
Jan 1, 1991·International Review of Cytology·P M Wassarman, S Mortillo
Jul 1, 1988·Developmental Biology·J Stewart-Savage, B D Bavister
Jan 1, 1988·Annual Review of Biochemistry·P M Wassarman
Jan 1, 1988·Journal of Reproduction and Fertility·R B Shabanowitz, M G O'Rand
Jun 1, 1987·Developmental Biology·J L Hedrick, N J Wardrip
Aug 15, 1986·Analytical Biochemistry·J L Hedrick, N J Wardrip
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk

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Citations

Feb 3, 2012·Cell and Tissue Research·Satish K GuptaNeha Gupta
Oct 21, 2011·Stroke Research and Treatment·Mark G BowdenChris M Gregory
Feb 3, 2004·Reproductive Biomedicine Online·Linda LefièvreStephen J Publicover

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