Feb 18, 2009

Composition of the immunoglobulin classic antigen-binding site regulates allergic airway inflammation in a murine model of experimental asthma

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
Sebastian KerzelMichael Zemlin

Abstract

When bound to mast cell FcepsilonRI, IgE serves as antigen receptor for allergic reactions, permitting specific identification of the allergen. Although the core of the classic antigen-binding site is heavy chain complementarity determining region 3 (CDR-H3), recent studies suggest that allergens might also bind IgE in a superantigen-like fashion outside the classic antigen-binding site. We sought to evaluate the contribution of the classic CDR-H3-centric antigen-binding site to the development of an allergic phenotype. Using a murine model of experimental asthma, we characterized a gene-targeted mouse strain expressing an altered range of CDR-H3s (DeltaD-iD mice) in response to the hydrophobic allergen ovalbumin (OVA). Mutant and wild-type (wt) mice were sensitized intraperitoneally with OVA; non-sensitized mice served as controls. We found the composition of the classic CDR-H3-centric antigen-binding site to be critical for the development of characteristic aspects of allergic asthma. (i) Compared with wt animals, DeltaD-iD mice showed a significantly less pronounced OVA-induced rise in allergen-specific IgE levels and hence in total serum IgE levels. (ii) In addition, DeltaD-iD mice demonstrated a significant reduction in eo...Continue Reading

  • References5
  • Citations34

References

Mentioned in this Paper

Eosinophil
Immune Response
Immunoglobulin Activity
Lung
Mast Cell
Receptors, Antigen
Murine
Recombinant Interleukin-5
SLC3A2 gene
Allergens

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.

Related Papers

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
J M Davies
Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
J M Davies, R E O'Hehir
Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
B M JensenL K Poulsen
© 2020 Meta ULC. All rights reserved