Compositional and abundance changes of nitrogen-cycling genes in plant-root microbiomes along a salt marsh chronosequence

Antonie van Leeuwenhoek
Miao WangJoana Falcão Salles

Abstract

Disentangling the relative influences of soil properties and plant-host on root-associated microbiomes in natural systems is challenging, given that spatially segregated soil types display distinct historical legacies. In addition, distant locations may also lead to biogeographical patterns of microbial communities. Here, we used an undisturbed salt marsh chronosequence spanning over a century of ecosystem development to investigate changes in the community composition and abundance of a set of nitrogen-cycling genes. Specifically, we targeted genes of diazotrophs and ammonia oxidizers associated with the bulk and rhizosphere soil of the plant species Limonium vulgare. Samples were collected across five distinct successional stages of the chronosequence (ranging from 5 to 105 years) at two time-points. Our results indicate that soil variables such as sand:silt:clay % content and pH strongly relates to the abundance of N-cycling genes in the bulk soil. However, in the rhizosphere samples, the abundance of ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively) was relatively constant across most of the successional stages, albeit displaying seasonal variation. This result indicates a potentially strong...Continue Reading

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Citations

Dec 22, 2019·Microorganisms·Jack K BeltzAnne E Bernhard
Apr 6, 2021·Frontiers in Microbiology·Qiong WangChunxiang Hu

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Methods Mentioned

BETA
PCR

Software Mentioned

GelCompar
vegan
R
PerMANOVA
RVAideMemoire
Hmisc
corrplot

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