Compositional and transcriptomic analysis associated with cuticle lipid production on rosette and inflorescence stem leaves in the extremophyte Thellungiella salsuginea

Physiologia Plantarum
Shuai TangXiaojing Xu

Abstract

The plant cuticle is a complex structure composed primarily of wax and cutin, but also contains cutan, glycerolipids, phenolics, polysaccharides and proteins. The cuticle plays an important protective role as barrier between plants and their environment. In this paper, 4-week-old leaves produced either on the rosette or on the inflorescence stem of the model extremophyte Thellungiella salsuginea were examined using scanning electron microscopy, cuticle permeability assays and chemical composition analysis. Results showed that stem leaves (SL) had more abundant cuticle lipids and lower cuticle permeability than rosette leaves (RL). SL were dominated by alkanes, especially the C29 and C31 homologs, whereas in RL the most abundant wax class was free very long-chain acids. The major cutin monomers for both leaf types were C18:2 dioic acids and 18-OH C18:2 acids. We performed Illumina high-throughput sequencing for SL and RL, and 3577 differentially expressed genes were identified. Sixty-five genes possibly involved in cuticular lipid biosynthesis, transport, or regulation was selected for further analysis. Many cuticle-associated genes exhibited differential expression levels that could be associated with compositional differences ...Continue Reading

References

Sep 18, 2001·Journal of Experimental Botany·M Riederer, L Schreiber
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 12, 2002·Annual Review of Plant Biology·Jian-Kang Zhu
May 1, 1995·Plant Physiology·M. A. JenksK. A. Feldmann
Dec 7, 2002·Progress in Lipid Research·L Kunst, A L Samuels
Feb 28, 2003·Journal of Plant Research·Sofia Loza-Cornejo, Teresa Terrazas
Sep 16, 2003·Current Opinion in Plant Biology·Kazuo ShinozakiMotoaki Seki
Oct 23, 2004·Science·Jamie A PighinA Lacey Samuels
Nov 8, 2005·Journal of Chemical Ecology·Caroline Müller, Markus Riederer
Apr 4, 2006·Current Opinion in Plant Biology·Christiane Nawrath
Jul 18, 2006·Nucleic Acids Research·Jia YeJun Wang
Oct 24, 2006·Phytochemistry·Isabel MolinaMike Pollard
Nov 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yonghua LiJohn Ohlrogge
Dec 14, 2007·Nucleic Acids Research·Minoru KanehisaYoshihiro Yamanishi
Apr 29, 2008·Trends in Plant Science·Mike PollardJohn B Ohlrogge
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Nov 19, 2008·Nature Reviews. Genetics·Zhong WangMichael Snyder
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Oct 13, 2009·Plant Physiology·Dylan K KosmaMatthew A Jenks
Oct 30, 2009·Current Opinion in Plant Biology·Ljerka Kunst, Lacey Samuels
Dec 5, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yonghua Li-BeissonFred Beisson
Jun 21, 2011·Plant Science : an International Journal of Experimental Plant Biology·Eva DomínguezAntonio Heredia
Sep 10, 2011·Genome Research·Sonia TarazonaAna Conesa

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Citations

Sep 25, 2019·International Journal of Molecular Sciences·Junqing HeXiaojing Xu
Aug 7, 2020·International Journal of Molecular Sciences·Zhenzhen ZhaoYe Xia

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