DOI: 10.1101/476309Nov 21, 2018Paper

Comprehensive analysis of miRNA and protein profiles within exosomes derived from canine lymphoid tumour cell lines

BioRxiv : the Preprint Server for Biology
Hajime AsadaHajime Tsujimoto

Abstract

Exosomes are small extracellular vesicles released from almost all cell types, which play roles in cell-cell communication. Recent studies have suggested that microenvironmental crosstalk mediated by exosomes is an important factor in the escape of tumour cells from the anti-tumour immune system in human haematopoietic malignancies. Here, we conducted comprehensive analysis of the miRNA and protein profiles within the exosomes released from four canine lymphoid tumour cell lines as a model of human lymphoid tumours. The results showed that the miRNAs and proteins abundantly contained in exosomes were similar among the four cell lines. However, the profiles of miRNA within exosomes differed among the cell lines and reflected the expression pattern of miRNAs of the parent cells. In the comparison of the amounts of miRNAs and proteins among the cell lines, those of three miRNAs (miR-151, miR-8908a-3p, and miR-486) and CD82 protein differed between exosomes derived from vincristine-sensitive and resistant cell lines. Further investigations are needed to elucidate the biological functions of the exosomal contents in the microenvironmental crosstalk of lymphoid tumours.

Related Concepts

Allergens
Canis familiaris
Cell Line, Tumor
tris(4,7-diphenyl-1,10-phenanthroline)ruthenium (II)
Hematopoietic Neoplasms
Tumor Cells, Uncertain Whether Benign or Malignant
Lymphoid Neoplasm
Analysis
MicroRNAs
Intercellular Communication Process

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