Comprehensive analysis of mycolic acid subclass and molecular species composition of Mycobacterium bovis BCG Tokyo 172 cell wall skeleton (SMP-105)

Journal of Microbiological Methods
Yuko UenishiMakoto Sunagawa

Abstract

The mycobacterial cell envelope consists of a characteristic cell wall skeleton (CWS), a mycoloyl arabinogalactan peptidoglycan complex, and related hydrophobic components that contribute to the cell surface properties. Since mycolic acids have recently been reported to play crucial roles in host immune response, detailed molecular characterization of mycolic acid subclasses and sub-subclasses of CWS from Mycobacterium bovis BCG Tokyo 172 (SMP-105) was performed. Mycolic acids were liberated by alkali hydrolysis from SMP-105, and their methyl esters were separated by silica gel TLC into three subclasses: alpha-, methoxy-, and keto-mycolates. Each mycolate subclass was further separated by silver nitrate (AgNO(3))-coated silica gel TLC into sub-subclasses. Molecular weights of individual mycolic acid were determined by MALDI-TOF mass spectrometry. alpha-Mycolates were sub-grouped into cis, cis-dicyclopropanoic (alpha1), and cis-monocyclopropanoic-cis-monoenoic (alpha2) series; methoxy-mycolates were sub-grouped into cis-monocyclopropanoic (m1), trans-monocyclopropanoic (m2), trans-monoenoic (m3), cis-monocyclopropanoic-trans-monoenoic (m4), cis-monoenoic (m5), and cis-monocyclopropanoic-cis-monoenoic (m6) series; and keto-mycola...Continue Reading

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Citations

Apr 23, 2013·Chemistry and Physics of Lipids·Mohammed O BalogunMark S Baird
Jun 5, 2012·Progress in Lipid Research·Jan A VerschoorJohan Grooten
Aug 31, 2012·Oncoimmunology·Lorenzo GalluzziGuido Kroemer
Jun 1, 2016·Nature Reviews. Microbiology·Andrea Du Toit
May 26, 2017·Critical Reviews in Analytical Chemistry·Nicoleta Elena DinaAlin Sebastian Porav
Sep 28, 2013·The Analyst·Omar E Rivera-BetancourtRichard A Dluhy
Feb 25, 2017·Nature Reviews. Microbiology·Elitza I TochevaGrant J Jensen
Nov 30, 2021·Journal of the American Society for Mass Spectrometry·Cheryl FrankfaterFong-Fu Hsu

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