Comprehensive annotation of Glossina pallidipes salivary gland hypertrophy virus from Ethiopian tsetse flies: a proteogenomics approach

The Journal of General Virology
Adly M M Abd-AllaMax Bergoin

Abstract

Glossina pallidipes salivary gland hypertrophy virus (GpSGHV; family Hytrosaviridae) can establish asymptomatic and symptomatic infection in its tsetse fly host. Here, we present a comprehensive annotation of the genome of an Ethiopian GpSGHV isolate (GpSGHV-Eth) compared with the reference Ugandan GpSGHV isolate (GpSGHV-Uga; GenBank accession number EF568108). GpSGHV-Eth has higher salivary gland hypertrophy syndrome prevalence than GpSGHV-Uga. We show that the GpSGHV-Eth genome has 190 291 nt, a low G+C content (27.9 %) and encodes 174 putative ORFs. Using proteogenomic and transcriptome mapping, 141 and 86 ORFs were mapped by transcripts and peptides, respectively. Furthermore, of the 174 ORFs, 132 had putative transcriptional signals [TATA-like box and poly(A) signals]. Sixty ORFs had both TATA-like box promoter and poly(A) signals, and mapped by both transcripts and peptides, implying that these ORFs encode functional proteins. Of the 60 ORFs, 10 ORFs are homologues to baculovirus and nudivirus core genes, including three per os infectivity factors and four RNA polymerase subunits (LEF4, 5, 8 and 9). Whereas GpSGHV-Eth and GpSGHV-Uga are 98.1 % similar at the nucleotide level, 37 ORFs in the GpSGHV-Eth genome had nucleotid...Continue Reading

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Citations

Sep 7, 2016·Journal of Invertebrate Pathology·Trevor WilliamsMonique M van Oers
Mar 30, 2017·Molecular Systems Biology·Pierre M Jean BeltranIleana M Cristea
Aug 8, 2019·The Journal of General Virology·Henry M Kariithi Ictv Report Consortium
Apr 25, 2018·Frontiers in Microbiology·Güler Demirbas-UzelAdly M M Abd-Alla
Jul 2, 2020·Infection and Drug Resistance·Ehsaneh KhodadadiHossein Samadi Kafil
Nov 25, 2018·BMC Microbiology·Henry M KariithiJust M Vlak
Mar 13, 2017·The Journal of General Virology·Annie BézierElisabeth A Herniou
Nov 25, 2018·BMC Microbiology·Irene K MekiAdly M M Abd-Alla
Aug 4, 2020·Computational Biology and Chemistry·Zhiyuan YangStephen Kwok-Wing Tsui
Oct 15, 2020·Archives of Virology·Norio MatsushimaRobert H Kretsinger

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