Comprehensive Autopsy Program for Individuals with Multiple Sclerosis.

Journal of Visualized Experiments : JoVE
Ranjan DuttaBruce D Trapp

Abstract

We describe a rapid tissue donation program for individuals with multiple sclerosis (MS) that requires scientists and technicians to be on-call 24/7, 365 days a year. Participants consent to donate their brain and spinal cord. Most patients were followed by neurologists at the Cleveland Clinic Mellen Center for MS Treatment and Research. Their clinical courses and neurological disabilities are well-characterized. Soon after death, the body is transported to the MS Imaging Center, where the brain is scanned in situ by 3 T magnetic resonance imaging (MRI). The body is then transferred to the autopsy room, where the brain and spinal cord are removed. The brain is divided into two hemispheres. One hemisphere is immediately placed in a slicing box and alternate 1 cm-thick slices are either fixed in 4% paraformaldehyde for two days or rapidly frozen in dry ice and 2-methylbutane. The short-fixed brain slices are stored in a cryopreservation solution and used for histological analyses and immunocytochemical detection of sensitive antigens. Frozen slices are stored at -80 °C and used for molecular, immunocytochemical, and in situ hybridization/RNA scope studies. The other hemisphere is placed in 4% paraformaldehyde for several months, ...Continue Reading

Citations

Apr 15, 2020·Annals of Neurology·Kedar R MahajanDaniel Ontaneda
Aug 21, 2020·Science·Fiachra HumphriesKatherine A Fitzgerald
May 13, 2021·Annals of Clinical and Translational Neurology·Ajai TripathiRanjan Dutta

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