Comprehensive Chromosome End Remodeling during Programmed DNA Elimination.

Current Biology : CB
Jianbin WangRichard E Davis

Abstract

Germline and somatic genomes are in general the same in a multicellular organism. However, programmed DNA elimination leads to a reduced somatic genome compared to germline cells. Previous work on the parasitic nematode Ascaris demonstrated that programmed DNA elimination encompasses high-fidelity chromosomal breaks and loss of specific genome sequences including a major tandem repeat of 120 bp and ~1,000 germline-expressed genes. However, the precise chromosomal locations of these repeats, breaks regions, and eliminated genes remained unknown. We used PacBio long-read sequencing and chromosome conformation capture (Hi-C) to obtain fully assembled chromosomes of Ascaris germline and somatic genomes, enabling a complete chromosomal view of DNA elimination. We found that all 24 germline chromosomes undergo comprehensive chromosome end remodeling with DNA breaks in their subtelomeric regions and loss of distal sequences including the telomeres at both chromosome ends. All new Ascaris somatic chromosome ends are recapped by de novo telomere healing. We provide an ultrastructural analysis of Ascaris DNA elimination and show that eliminated DNA is incorporated into double membrane-bound structures, similar to micronuclei, during telo...Continue Reading

References

Jul 1, 1992·The Journal of Cell Biology·C GodayS Pimpinelli
Jan 1, 1985·Chromosoma·C GodayS Pimpinelli
Jan 1, 1996·Journal of Structural Biology·J R KremerJ R McIntosh
Jan 27, 1998·Journal of Structural Biology·D N Mastronarde
Dec 16, 1998·Science·UNKNOWN C. elegans Sequencing Consortium
May 2, 2002·Cell·Craig H BassingFrederick W Alt
Oct 1, 2003·Journal of Microscopy·T Müller-ReichertK McDonald
Nov 12, 2003·The Journal of Cell Biology·Eileen T O'TooleThomas Müller-Reichert
Jun 18, 2005·Bioinformatics·Alkes L PricePavel A Pevzner
Sep 27, 2005·Journal of Structural Biology·David N Mastronarde
Mar 23, 2006·Annual Review of Immunology·David JungFrederick W Alt
Sep 18, 2007·Biochimie·Alexandre OttavianiFrédérique Magdinier
Sep 22, 2007·Science·Elodie GhedinAlan L Scott
Jan 16, 2008·BMC Bioinformatics·David EllinghausUte Willhoeft
Jan 30, 2008·Cell Structure and Function·Hiroshi KimuraNaohito Nozaki
Mar 31, 2010·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Noriko F KojimaSouichirou Kubota
Jul 24, 2010·Genome Research·Henrik Kaessmann
Aug 23, 2011·Annual Review of Genetics·David G Schatz, Patrick C Swanson
Mar 1, 2012·Eukaryotic Cell·Ming-Liang Liu, Meng-Chao Yao
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Jul 24, 2012·Current Biology : CB·Jeramiah J SmithChris T Amemiya
Nov 6, 2012·Developmental Cell·Jianbin WangRichard E Davis
Feb 5, 2013·Cell·John R BrachtLaura F Landweber
Mar 26, 2013·Nature Genetics·Christopher A DesjardinsThomas B Nutman
Jun 4, 2013·The Biochemical Journal·Qun LuHong Zhang
Sep 27, 2013·Nucleic Acids Research·Wil A M LoenenGeoffrey G Wilson
Jun 3, 2014·Current Opinion in Genetics & Development·Jianbin Wang, Richard E Davis
Oct 18, 2014·Molecular Human Reproduction·Steven A RammJoachim Wistuba

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Citations

Feb 10, 2021·G3 : Genes - Genomes - Genetics·Pablo Manuel Gonzalez de la RosaMark Blaxter
Feb 22, 2021·Current Opinion in Cell Biology·Ksenia KrupinaDon W Cleveland
Sep 9, 2020·Current Biology : CB·Kazufumi Mochizuki
Apr 24, 2021·Genome Biology and Evolution·Christina N Hodson, Laura Ross
Sep 21, 2021·Biological Reviews of the Cambridge Philosophical Society·Dmitrij Dedukh, Alla Krasikova

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