Comprehensive comparison of liquid chromatography selectivity as provided by two types of liquid chromatography detectors (high resolution mass spectrometry and tandem mass spectrometry): "where is the crossover point?"

Analytica Chimica Acta
A KaufmannM Widmer

Abstract

The selectivity of mass traces obtained by monitoring liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS) and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was compared. A number of blank extracts (fish, pork kidney, pork liver and honey) were separated by ultra performance liquid chromatography (UPLC). Detected were some 100 dummy transitions respectively dummy exact masses (traces). These dummy masses were the product of a random generator. The range of the permitted masses corresponded to those which are typical for analytes (e.g. veterinary drugs). The large number of monitored dummy traces ensured that endogenous compounds present in the matrix extract, produced a significant number of detectable chromatographic peaks. All obtained chromatographic peaks were integrated and standardized. Standardisation was done by dividing these absolute peak areas by the average response of a set of 7 different veterinary drugs. This permitted a direct comparison between the LC-HRMS and LC-MS/MS data. The data indicated that the selectivity of LC-HRMS exceeds LC-MS/MS, if high resolution mass spectrometry (HRMS) data is recorded with a resolution of 50,000 full width at half maximum (FWHM) a...Continue Reading

References

Oct 11, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·David J FoltzTimothy R Baker
Nov 9, 2006·Rapid Communications in Mass Spectrometry : RCM·Anton Kaufmann, Patrick Butcher
Feb 26, 2009·Rapid Communications in Mass Spectrometry : RCM·Anton KaufmannDiana Uría

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Citations

Dec 20, 2011·Analytical and Bioanalytical Chemistry·Anton Kaufmann
Aug 9, 2011·Environmental Science and Pollution Research International·Andrés Pérez-ParadaAmadeo R Fernández-Alba
Mar 5, 2013·Environmental Science and Pollution Research International·Ana AgüeraAmadeo R Fernández-Alba
Jun 20, 2012·Journal of the American Society for Mass Spectrometry·Timothy R CroleySteven M Musser
Mar 11, 2011·Journal of Agricultural and Food Chemistry·Sergio C NanitaJohn H May
Oct 20, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Michael S FiligenziRobert H Poppenga
Feb 13, 2016·Rapid Communications in Mass Spectrometry : RCM·Takao SanakiYoshito Numata
Dec 25, 2015·Computational and Structural Biotechnology Journal·Shuzhao LiRuiyan Luo
Mar 2, 2016·Journal of Pharmaceutical and Biomedical Analysis·Baptiste GrundBertrand Rochat
Sep 25, 2014·Mass Spectrometry Reviews·Michel WagnerGérard Hopfgartner
Sep 14, 2012·Journal of Mass Spectrometry : JMS·Guglielmo DusiRoberta Galarini
Nov 14, 2012·Journal of Mass Spectrometry : JMS·Juan Antonio Padilla-SánchezAntonia Garrido-Frenich
Dec 3, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Noelia López-GutiérrezAntonia Garrido Frenich
Sep 24, 2016·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Vaishali BaneAmbrose Furey

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