Comprehensive construction strategy of bidirectional green tissue-specific synthetic promoters

Plant Biotechnology Journal
Jiuyuan BaiRui Wang

Abstract

Bidirectional green tissue-specific promoters have important application prospects in genetic engineering and crop genetic improvement. However, there is no report on the application of them, mainly due to undiscovered natural bidirectional green tissue-specific promoters and the lack of a comprehensive approach for the synthesis of these promoters. In order to compensate for this vacancy, the present study reports a novel strategy for the expression regulatory sequence selection and the bidirectional green tissue-specific synthetic promoter construction. Based on this strategy, seven promoters were synthesized and introduced into rice by agrobacterium-mediated transformation. The functional identification of these synthetic promoters was performed by the expression pattern of GFP and GUS reporter genes in two reverse directions in transgenic rice. The results indicated that all the synthetic promoters possessed bidirectional expression activities in transgenic rice, and four synthetic promoters (BiGSSP2, BiGSSP3, BiGSSP6, BiGSSP7) showed highly bidirectional expression efficiencies specifically in green tissues (leaf, sheath, panicle, stem), which could be widely applied to agricultural biotechnology. Our study provided a feas...Continue Reading

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Citations

Apr 24, 2021·Transgenic Research·S Selma, D Orzáez
May 1, 2021·Current Opinion in Plant Biology·Debao HuangWusheng Liu

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Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

Leica Application Suite

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