Comprehensive data analysis for development of custom qRT-PCR miRNA assay for glioblastoma: a prevalidation study

Epigenomics
Eman A ToraihManal S Fawzy

Abstract

Glioblastoma (GB) is one notable example of miRNA-modulated neoplasms. Given its unique expression signature, proper miRNA profiling can help discriminate between GB and other types of brain tumors. The current work aimed to develop a more GB-specific and applicable custom designed quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) miRNA assay. A comprehensive data analysis of bioinformatics databases, previous literature and commercially available pre-designed miRNA PCR arrays within the market. A highly enriched panel of 84 deregulated and GB-specific miRNAs has been developed. After validation of this newly developed array, it can not only save the researcher's time and effort, but can also have a potential diagnostic and/or prognostic role in GB, paving the road toward personalized medicine.

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Citations

May 31, 2019·Journal of Experimental & Clinical Cancer Research : CR·George E D PetrescuMihnea P Dragomir
Aug 3, 2021·Frontiers in Bioengineering and Biotechnology·Xiping HeZhong Feng

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Datasets Mentioned

BETA
GSE25631
GSE1330

Methods Mentioned

BETA
PCR

Software Mentioned

miProfile
DIANA
miRTar
Google Scholar
miRPath
Limma
DIANA miRPath
R
DIANA Tools Online Suite ( DIANA - microT - CDS

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