Comprehensive DNA Methylation Analysis of Retrotransposons in Male Germ Cells

Cell Reports
Ippei NagamoriToru Nakano

Abstract

De novo DNA methylation of retrotransposons is critical for silencing. Here, we use DNA methylation analysis to examine retrotransposons in mouse male germ cells. DNA methylation of long interspersed nuclear elements (LINEs) is dependent on piRNA, and younger LINEs exhibit greater piRNA dependence. In contrast, most long terminal repeat (LTR) retrotransposons produce lower levels of piRNAs and do not show significant piRNA dependence. The relationship between DNA methylation and corresponding piRNA expression of several LTR retrotransposons was reduced in Mili-null cells, but not Miwi2-null cells. These observations raise the possibility of piRNA-dependent DNA methylation without Miwi2. Therefore, it appears that the molecular mechanisms of the gene silencing of retrotransposons are more complicated than previously thought.

References

Oct 9, 2002·Genome Research·Prescott L Deininger, Mark A Batzer
Dec 6, 2002·Nature·UNKNOWN Mouse Genome Sequencing ConsortiumEric S Lander
Aug 20, 2004·Nature·Déborah Bourc'his, Timothy H Bestor
Mar 31, 2007·Developmental Cell·Michelle A CarmellGregory J Hannon
Apr 21, 2007·Science·Alexei A AravinGregory J Hannon
Mar 28, 2008·Molecular Biology and Evolution·Kohji Okamura, Kenta Nakai
Mar 10, 2009·Cold Spring Harbor Symposia on Quantitative Biology·A A Aravin, G J Hannon
Mar 24, 2009·Current Opinion in Cell Biology·Mikiko C Siomi, Satomi Kuramochi-Miyagawa
May 5, 2010·Genes & Development·Satomi Kuramochi-MiyagawaToru Nakano
Jun 1, 2012·Nucleic Acids Research·Fumihito MiuraTakashi Ito
Jan 5, 2013·Mobile DNA·Akash SookdeoStéphane Boissinot
Jun 7, 2014·Cell Stem Cell·Heather J LeeWolf Reik
Jul 18, 2014·Genes & Development·Antoine MolaroGregory J Hannon

❮ Previous
Next ❯

Citations

Aug 19, 2015·Cell Reports·Sergei A ManakovAlexei A Aravin
Oct 4, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hisato KobayashiTomohiro Kono
Apr 6, 2018·Animal : an International Journal of Animal Bioscience·Jeremy F TaylorPeter Sutovsky
Aug 27, 2017·RNA·Ildar GainetdinovTatyana Azhikina
May 10, 2020·Genes & Development·Fang YangP Jeremy Wang
Nov 18, 2018·Nature Reviews. Genetics·Deniz M OzataPhillip D Zamore
Nov 12, 2017·Nature Communications·Christina ErnstClaudia Kutter
Mar 7, 2020·Epigenetics & Chromatin·Iyer Aditya MahadevanManchanahalli R Satyanarayana Rao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.