Comprehensive evaluation of SCFA production in the intestinal bacteria regulated by berberine using gas-chromatography combined with polymerase chain reaction

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Lu-Lu WangJian-Dong Jiang

Abstract

Short-chain fatty acids (SCFAs) of intestine microbial have caught accumulating attention for their beneficial effects on human health. Botanic compounds with low bioavailability such as berberine (BBR) and resveratrol might interact with intestinal microbial ecosystem and promote gut bacteria to produce SCFA, which contribute to their biological effects. In the present study, a comprehensive assay system was built to detect SCFAs production in intestinal bacteria, in which stringent anaerobic culture was applied for in vitro bacterial fermentation, followed by direct-injection GC detection (chemical detection) in combination with real time polymerase chain reaction (RT-PCR, biological detection). BBR was used as positive reference. The direct injection GC method was calibrated and successfully applied to analyze the concentration of SCFAs in gut microbiota and BBR was proved to be effective in the dose- and time-dependent up-regulation of SCFAs production. As compared to the saline group, the concentration of acetic acid, propionate acid and butyric acid (the main SCFAs in gut microbiota) were increased by 17.7%, 11.1% and 30.5%, respectively, after incubating intestinal bacteria with 20μg/mL BBR for 24h. The increase reached ...Continue Reading

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Citations

Aug 20, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mengfan YueYue Dai
May 26, 2020·Journal of Food Science·Chung-Yi WangChih-Yao Hou
Oct 1, 2020·The Journal of Microbiology·Renbin LinShujie Chen
Dec 12, 2018·Frontiers in Pharmacology·Wuwen FengCheng Peng
Apr 2, 2019·Frontiers in Nutrition·Veronica LazarMariana Carmen Chifiriuc
Jul 6, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Jindong ZhaoZhaohui Fang
Apr 12, 2019·Analytical and Bioanalytical Chemistry·Shu-Rong MaJian-Dong Jiang
Oct 16, 2019·Journal of Pharmaceutical and Biomedical Analysis·Jessica FioriRoberto Gotti
Mar 9, 2021·Frontiers in Cellular and Infection Microbiology·Lichao ZhangLifu Wang
Mar 16, 2021·World Journal of Gastroenterology : WJG·Jin-Dong ZhaoZhao-Hui Fang

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