Comprehensive Identification and Bread-Making Quality Evaluation of Common Wheat Somatic Variation Line AS208 on Glutenin Composition

PloS One
Huiyun LiuXingguo Ye

Abstract

High molecular weight glutenin subunits (HMW-GSs) are important seed storage proteins in wheat (Triticum aestivum) that determine wheat dough elasticity and processing quality. Clarification of the defined effectiveness of HMW-GSs is very important to breeding efforts aimed at improving wheat quality. To date, there have no report on the expression silencing and quality effects of 1Bx20 and 1By20 at the Glu-B1 locus in wheat. A wheat somatic variation line, AS208, in which both 1Bx20 and 1By20 at Glu-B1 locus were silenced, was developed recently in our laboratory. Evaluation of agronomic traits and seed storage proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and reversed-phase high performance liquid chromatography (RP-HPLC) indicated that AS208 was highly similar to its parental cultivar Lunxuan987 (LX987), with the exception that the composition and expression of HMW-GSs was altered. The 1Bx20 and 1By20 in AS208 were further identified to be missing by polymerase chain reaction (PCR) and quantitative real-time RT-PCR (qRT-PCR) assays. Based on the PCR results for HMW-GS genes and their promoters in AS208 compared with LX987, 1Bx20 and 1By20 were speculated to be deleted in AS208 during in vit...Continue Reading

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Citations

Mar 14, 2018·Journal of the Science of Food and Agriculture·Jiaxing ZhouYueming Yan
Dec 11, 2019·Plant Molecular Biology·Luning DuanXiaohui Li
Sep 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Fatma BoukidBarbara Prandi
Dec 31, 2020·International Journal of Molecular Sciences·Yi LiDong Yang

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Datasets Mentioned

BETA
AS208

Methods Mentioned

BETA
electrophoresis
reverse transcription PCR
PCR
transgenic

Software Mentioned

ABI 7500
SPSS

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