Comprehensive imaging of porcine adrenal gland lipids by MALDI-FTMS using quercetin as a matrix

Analytical Chemistry
Xiaodong WangChristoph H Borchers

Abstract

Adrenal glands synthesize and release functional zone-specific steroid and catecholamine hormones to regulate mammalian stress responses. Lipids such as sphingolipids have been shown to control the steroid hormone biosynthesis in adrenal glands, indicating their important roles in endocrine organs. Molecular imaging by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a well-established analytical technique for determining both the spatial location and the relative abundances of various lipids on tissue. To better understand the overall roles of different lipid classes that play in the mammalian adrenal glands, it is necessary to comprehensively determine the spatial distributions of various lipids in the different functional zones of adrenal glands. However, the potential of this technique has not been fully reached, considering there are thousands of lipid species in a cell or tissue. To achieve this, we used quercetin as a MALDI matrix for negative ion detection of endogenous lipids on tissue sections of porcine adrenal glands by MALDI-Fourier-transform ion cyclotron resonance (FTICR) MS. As a result of these experiments, 409 endogenous compounds were detected in the negative ion mode. Combining bot...Continue Reading

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Citations

Aug 16, 2015·Journal of Chromatography. a·Núria CanelaLluís Arola
Apr 24, 2015·Journal of the American Society for Mass Spectrometry·Jeffrey M SpragginsRichard M Caprioli
Jan 27, 2015·Laboratory Investigation; a Journal of Technical Methods and Pathology·Michaela Aichler, Axel Walch
Oct 1, 2016·Current Opinion in Biotechnology·Teesha C BakerChristoph H Borchers
Feb 7, 2019·Histochemistry and Cell Biology·Thomas G PapathomasAxel Karl Walch
Apr 24, 2018·Analytical and Bioanalytical Chemistry·Cosima Damiana CalvanoFrancesco Palmisano
Jan 1, 2015·Chemical Science·Xiaodong WangChristoph H Borchers
Jul 30, 2014·Analytical Chemistry·E Ellen JonesRichard R Drake

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