Comprehensive in vitro and in vivo studies of novel melt-derived Nb-substituted 45S5 bioglass reveal its enhanced bioactive properties for bone healing

Scientific Reports
Lucas SouzaCelso Aparecido Bertran

Abstract

The present work presents and discusses the results of a comprehensive study on the bioactive properties of Nb-substituted silicate glass derived from 45S5 bioglass. In vitro and in vivo experiments were performed. We undertook three different types of in vitro analyses: (i) investigation of the kinetics of chemical reactivity and the bioactivity of Nb-substituted glass in simulated body fluid (SBF) by 31P MASNMR spectroscopy, (ii) determination of ionic leaching profiles in buffered solution by inductively coupled plasma optical emission spectrometry (ICP-OES), and (iii) assessment of the compatibility and osteogenic differentiation of human embryonic stem cells (hESCs) treated with dissolution products of different compositions of Nb-substituted glass. The results revealed that Nb-substituted glass is not toxic to hESCs. Moreover, adding up to 1.3 mol% of Nb2O5 to 45S5 bioglass significantly enhanced its osteogenic capacity. For the in vivo experiments, trial glass rods were implanted into circular defects in rat tibia in order to evaluate their biocompatibility and bioactivity. Results showed all Nb-containing glass was biocompatible and that the addition of 1.3 mol% of Nb2O5, replacing phosphorous, increases the osteostimul...Continue Reading

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Citations

Aug 24, 2019·Nanoscale·Filipe V FerreiraOrlando J Rojas
Oct 28, 2019·Journal of Biomedical Materials Research. Part a·Lucas P L de SouzaJosé A Camilli
Mar 4, 2020·International Journal of Molecular Sciences·Fabian WesthauserAldo R Boccaccini
Oct 5, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·João H LopesCelso A Bertran
Mar 31, 2021·The Journal of Foot and Ankle Surgery : Official Publication of the American College of Foot and Ankle Surgeons·Hongdong MaMaowei Yang
Apr 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Filipe V FerreiraLuiz H C Mattoso
May 6, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Kuei-Chih FengPin-Yi Chen
Dec 24, 2021·Journal of Materials Science. Materials in Medicine·Usanee PantulapAldo R Boccaccini

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Methods Mentioned

BETA
NMR
scanning electron microscopy

Software Mentioned

NIS
TopSpin
Elements software Advanced Research

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