Comprehensive investigation of a dye-decolorizing peroxidase and a manganese peroxidase from Irpex lacteus F17, a lignin-degrading basidiomycete

AMB Express
Zihong DuanRong Jia

Abstract

Irpex lacteus F17 is well-known for its ability to degrade recalcitrant aromatic pollutants, which mainly results from the action of the manganese peroxidase (MnP) that it is able to produce. Recently, the genome sequencing and annotation of this strain provided comprehensive picture of the ligninolytic peroxidase gene family. In addition to revealing the presence of 13 MnPs, genes for five dye-decolorizing peroxidases (DyPs) were also discovered in the I. lacteus F17 genome, which are unrelated to the fungal class II peroxidases. In the present study, amino acid sequences of five DyPs and 13 MnPs, representing two different families of heme peroxidases, were analyzed. Of these, two enzymes, a DyP (Il-DyP4) and a MnP (Il-MnP6) were expressed respectively in Escherichia coli, and were characterized by comparing their molecular models, substrate specificities, and catalytic features. The results showed that Il-DyP4 possessed a higher catalytic efficiency for some representative substrates, and a stronger decolorizing ability to a wide range of synthetic dyes in acidic conditions. Based on electrochemical measurements, Il-DyP4 was found to have a high redox potential of 27 mV at pH 3.5, which was superior to that of Il-MnP6 (- 75 ...Continue Reading

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Citations

Apr 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nina-Katharina KraheFranziska Ersoy
Aug 30, 2020·Biotechnology and Applied Biochemistry·Gianluca CatucciGianfranco Gilardi
Dec 29, 2020·The FEBS Journal·Ulises A ZitareMarco W Fraaije
Dec 2, 2020·Bioresource Technology·Zhaoxian XuMingjie Jin
Sep 25, 2020·Archives of Biochemistry and Biophysics·Poonam DhankharPravindra Kumar
Apr 28, 2021·Proceedings of the National Academy of Sciences of the United States of America·Nicola C OatesNeil C Bruce
Jun 3, 2021·International Journal of Molecular Sciences·Yasushi Sugano, Toru Yoshida

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Datasets Mentioned

BETA
JQ403610.3.2
MG
209114
209112
AF
2014020
MQVO00000000
BAA77283.1
CAK55151.1
EIW57847.1

Methods Mentioned

BETA
PCR
electrophoresis
Circular dichroism

Software Mentioned

SWISS MODEL
adusta
Protein Blast
PyMOL
Clustal X
ExPASy
K2D
SignalP

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