Comprehensive map and functional annotation of the mouse white adipose tissue proteome

PeerJ
Xiaoyue TangWei Sun

Abstract

White adipose tissue (WAT) plays a significant role in energy metabolism and the obesity epidemic. In this study, we sought to (1) profile the mouse WAT proteome with advanced 2DLC/MS/MS approach, (2) provide insight into WAT function based on protein functional annotation, and (3) predict potentially secreted proteins. A label-free 2DLC/MS/MS proteomic approach was used to identify the WAT proteome from female mouse WAT. A total of 6,039 proteins in WAT were identified, among which 5,160 were quantified (spanning a magnitude of 106) using an intensity-based absolute quantification algorithm, and 3,117 proteins were reported by proteomics technology for the first time in WAT. To comprehensively analyze the function of WAT, the proteins were divided into three quantiles based on abundance and we found that proteins of different abundance performed different functions. High-abundance proteins (the top 90%, 1,219 proteins) were involved in energy metabolism; middle-abundance proteins (90-99%, 2,273 proteins) were involved in the regulation of protein synthesis; and low-abundance proteins (99-100%, 1,668 proteins) were associated with lipid metabolism and WAT beiging. Furthermore, 800 proteins were predicted by SignalP4.0 to have s...Continue Reading

References

Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·L PereiraF Ramirez
Apr 27, 1999·The International Journal of Biochemistry & Cell Biology·S R Kimball
Feb 1, 2000·Journal of Bioenergetics and Biomembranes·M Klingenberg
Oct 30, 2001·The Proceedings of the Nutrition Society·P Trayhurn, J H Beattie
Jan 17, 2003·Molecular and Cellular Biology·Leanne Wilson-FritchSilvia Corvera
Jan 13, 2004·Physiological Reviews·Barbara Cannon, Jan Nedergaard
Apr 28, 2004·Biochemical and Biophysical Research Communications·I P LópezC De Miguel
Jun 29, 2004·The Journal of Biological Chemistry·Krishnakant G SoniGrant A Mitchell
Oct 8, 2004·The British Journal of Nutrition·Paul Trayhurn, I Stuart Wood
Oct 1, 2005·Analytical Chemistry·Martin GilarJohn C Gebler
Apr 29, 2006·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Dominique Langin
Apr 6, 2007·Molecular & Cellular Proteomics : MCP·Jun AdachiMatthias Mann
Dec 18, 2008·Expert Review of Proteomics·Xiaoli Chen, Sonja Hess
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Aug 25, 2009·The American Journal of Pathology·Aurélia De PauwThierry Arnould
May 20, 2011·Nature·Björn SchwanhäusserMatthias Selbach
Sep 13, 2011·International Journal of Cardiology·Aristeidis G VaiopoulosMichael Koutsilieris
Sep 8, 2012·Nature·UNKNOWN ENCODE Project Consortium
Jul 23, 2013·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Lucila Sackmann-SalaJohn J Kopchick
Oct 9, 2013·Nature Medicine·Matthew Harms, Patrick Seale
Nov 28, 2013·Clinics in Liver Disease·Christine McGownZobair M Younossi
May 30, 2014·Nature·Min-Sik KimAkhilesh Pandey
May 30, 2014·Nature·Mathias WilhelmBernhard Kuster
Aug 8, 2014·Peptides·João Marcus Oliveira AndradeSérgio Henrique Sousa Santos
Aug 17, 2014·Experimental Physiology·Sihem Boudina, Timothy E Graham
Mar 21, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Sang Woo KimJong Won Yun
Jun 11, 2015·Trends in Endocrinology and Metabolism : TEM·Ashley S WilliamsDavid H Wasserman

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Methods Mentioned

BETA
electrophoresis
ubiquitination

Software Mentioned

Proteome
IPA
Scaffold
Progenesis
PANTHER
iBAQ
Mascot
SignalP

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