Comprehensive mass spectrometry-guided phenotyping of plant specialized metabolites reveals metabolic diversity in the cosmopolitan plant family Rhamnaceae

The Plant Journal : for Cell and Molecular Biology
Kyo Bin KangPieter C Dorrestein

Abstract

Plants produce a myriad of specialized metabolites to overcome their sessile habit and combat biotic as well as abiotic stresses. Evolution has shaped the diversity of specialized metabolites, which then drives many other aspects of plant biodiversity. However, until recently, large-scale studies investigating the diversity of specialized metabolites in an evolutionary context have been limited by the impossibility of identifying chemical structures of hundreds to thousands of compounds in a time-feasible manner. Here we introduce a workflow for large-scale, semi-automated annotation of specialized metabolites and apply it to over 1000 metabolites of the cosmopolitan plant family Rhamnaceae. We enhance the putative annotation coverage dramatically, from 2.5% based on spectral library matches alone to 42.6% of total MS/MS molecular features, extending annotations from well-known plant compound classes into dark plant metabolomics. To gain insights into substructural diversity within this plant family, we also extract patterns of co-occurring fragments and neutral losses, so-called Mass2Motifs, from the dataset; for example, only the Ziziphoid clade developed the triterpenoid biosynthetic pathway, whereas the Rhamnoid clade predo...Continue Reading

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Citations

Jan 11, 2020·The Plant Journal : for Cell and Molecular Biology·Paul D FraserAlisdair R Fernie
Dec 12, 2019·Natural Product Reports·Marc G ChevretteFrancisco Barona-Gómez
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Jun 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kwangho SongIn Jin Ha
Mar 7, 2021·Metabolites·Karen McLuskeyRónán Daly

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