Comprehensive metabolomic profiling of osteosarcoma based on UHPLC-HRMS.

Metabolomics : Official Journal of the Metabolomic Society
Dongming LvXianbiao Xie

Abstract

Osteosarcoma (OS) is the most common primary malignant bone tumor in children and adolescents. An increasing number of studies have demonstrated that tumor proliferation and metastasis are closely related to complex metabolic reprogramming. However, there are limited data to provide a comprehensive metabolic picture of osteosarcoma. Our study aims to identify aberrant metabolic pathways and seek potential adjuvant biomarkers for osteosarcoma. Serum samples were collected from 65 osteosarcoma patients and 30 healthy controls. Nontargeted metabolomic profiling was performed by liquid chromatography-mass spectrometry (LC-MS) based on univariate and multivariate statistical analyses. The OPLS-DA model analysis identified clear separations among groups. We identified a set of differential metabolites such as higher serum levels of adenosine-5-monophosphate, inosine-5-monophosphate and guanosine monophosphate in primary OS patients compared to healthy controls, and higher serum levels of 5-aminopentanamide, 13(S)-HpOTrE (FA 18:3 + 2O) and methionine sulfoxide in lung metastatic OS patients compared to primary OS patients, revealing aberrant metabolic features during the proliferation and metastasis of osteosarcoma. We found a group o...Continue Reading

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Citations

Jul 3, 2021·Cells·Miguel Esperança-MartinsSérgio Dias
Aug 7, 2021·Metabolites·Jingzhi FanKristaps Klavins

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Methods Mentioned

BETA
metabolomic profiling
metabolomics profiling

Software Mentioned

OPLS
CAMERA
MultiExperiment Viewer ( Mev
ProteoWizard
SIMCA
R
DA
XCMS
GraphPad Prism
SPSS

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