Comprehensive molecular characterization of microneedling therapy in a human three-dimensional skin model

PloS One
Laurenz SchmittJens-Malte Baron

Abstract

Microneedling therapy is a widely used technique in dermatology. However, little is known about the underlying molecular effects of this therapy on extracellular matrix remodeling, wound healing, and inflammation. The aim of this study was to examine morphological and molecular changes caused by microneedling treatment in a standardized in vitro full-thickness 3D model of human skin. A microneedling device was used to treat full-thickness 3D skin models. Specimens were harvested at specified time points and qRT-PCR and microarray studies were performed. Frozen sections were examined histologically. Microneedling treatment caused morphological changes in the skin model resulting in an almost complete recovery of the epidermis five days after treatment. Microarray analysis identified an upregulation of genes that are associated with tissue remodeling and wound healing (e.g. COL3A1, COL8A1, TIMP3), epithelial proliferation and differentiation (KRT13, IGF1), immune cell recruitment (CCL11), and a member of the heat shock protein family (HSPB6). On the other hand, we detected a downregulation of pro-inflammatory cytokines (e.g. IL1α, IL1β, IL24, IL36γ, IL36RN), and antimicrobial peptides (e.g. S100A7A, DEFB4). These data were confir...Continue Reading

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Citations

Jun 11, 2020·Journal of Cosmetic Dermatology·Michael H GoldJanelle H Vega
Jul 13, 2020·Dermatologic Therapy·Master Zduńska-Pęciak Kamila, Rotsztejn Helena
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Mar 13, 2021·Annals of Maxillofacial Surgery·Enosh Nirmalkumar StewardKartik Dholakia
Jun 29, 2021·Plastic and Reconstructive Surgery·Mimi R BorrelliH Peter Lorenz

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Methods Mentioned

BETA
biopsies
biopsy
Chip
PCR

Software Mentioned

GraphPad PRISM
- SpringGX
Gene

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