Comprehensive multiphase NMR applied to a living organism

Chemical Science
Yalda Liaghati MobarhanAndre J Simpson

Abstract

Comprehensive multiphase (CMP) NMR is a novel technology that integrates all the hardware from solution-, gel- and solid-state into a single NMR probe, permitting all phases to be studied in intact samples. Here comprehensive multiphase (CMP) NMR is used to study all components in a living organism for the first time. This work describes 4 new scientific accomplishments summarized as: (1) CMP-NMR is applied to a living animal, (2) an effective method to deliver oxygen to the organisms is described which permits longer studies essential for in-depth NMR analysis in general, (3) a range of spectral editing approaches are applied to fully differentiate the various phases solutions (metabolites) through to solids (shell) (4) 13C isotopic labelling and multidimensional NMR are combined to provide detailed assignment of metabolites and structural components in vivo. While not explicitly studied here the multiphase capabilities of the technique offer future possibilities to study kinetic transfer between phases (e.g. nutrient assimilation, contaminant sequestration), molecular binding at interfaces (e.g. drug or contaminant binding) and bonding across and between phases (e.g. muscle to bone) in vivo. Future work will need to focus on ...Continue Reading

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Citations

Jun 25, 2017·Photosynthesis Research·Fatemeh Azadi-ChegeniAnjali Pandit
Apr 3, 2020·Magnetic Resonance in Chemistry : MRC·Ronald SoongAndre Simpson
Jul 22, 2020·The Analyst·Maryam Tabatabaei AnarakiAndré J Simpson
May 23, 2019·Frontiers in Molecular Biosciences·Michael T JudgeArthur S Edison
Apr 6, 2017·Angewandte Chemie·Ioana FugariuAndre J Simpson
Aug 15, 2018·Analytical and Bioanalytical Chemistry·Paris NingAndré J Simpson
Jan 3, 2019·Journal of Biomolecular NMR·Daniel LaneAndré J Simpson
Mar 15, 2019·Metabolomics : Official Journal of the Metabolomic Society·Henrik Max Jensen, Hanne Christine Bertram
Nov 25, 2017·The Analyst·I FugariuA J Simpson
Apr 3, 2020·Magnetic Resonance in Chemistry : MRC·Yalda Liaghati MobarhanAndre J Simpson
Jun 25, 2017·Analytical and Bioanalytical Chemistry·Yalda Liaghati MobarhanAndré J Simpson
Jun 16, 2018·Magnetic Resonance in Chemistry : MRC·Maryam Tabatabaei AnarakiAndré J Simpson
Aug 10, 2021·NMR in Biomedicine·Amith Sadananda Maroli, Robert Powers
Nov 13, 2020·Analytical Chemistry·Arthur S EdisonSicong Zhang
Jun 5, 2018·Analytical Chemistry·Maryam Tabatabaei AnarakiAndré J Simpson
Jul 21, 2017·Journal of Agricultural and Food Chemistry·Blythe E Fortier-McGillAndré J Simpson
Nov 11, 2020·Analytical Chemistry·Vincent Moxley-PaquetteAndré J Simpson

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Methods Mentioned

BETA
NMR
deamination
environmental stress

Software Mentioned

PHORMAT
AMIX ( Analysis of MIXtures

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