Comprehensive proteomic and metabolomic profiling of mcr-1-mediated colistin resistance in Escherichia coli

International Journal of Antimicrobial Agents
Hui LiJianzhong Shen

Abstract

Spread of the mcr-1 gene in human and veterinary medicine has jeopardised the use of polymyxins, last-resort antibiotics against life-threatening multidrug-resistant Gram-negative bacteria. As a lipid-modifying gene, whether mcr-1 causes proteomic and metabolomic changes in bacteria and affects the corresponding metabolic pathway is largely unknown. In this study, label-free quantitative proteomics and untargeted metabolomics were used to profile comprehensive proteome and metabolome characteristics of mcr-1-mediated colistin-resistant and -susceptible Escherichia coli in order to gain further insight into the colistin resistance mechanism. Large sets of differentially expressed proteins (DEPs) and metabolites were identified that contributed to mcr-1-mediated antimicrobial resistance, predominantly in different growth conditions with and without colistin. mcr-1 caused downregulated expression of most proteins in order to adapt to drug pressure. Pathway analysis showed that metabolic processes were significantly affected, mainly related to glycerophospholipid metabolism, thiamine metabolism and lipopolysaccharide (LPS) biosynthesis. The substrate phosphoethanolamine (PEA) for mcr-1 to mediate colistin resistance was accumulated...Continue Reading

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Citations

Jun 10, 2020·Infection and Immunity·Giorgio MattiuzMaria Gabriella Torcia
Jul 2, 2020·Infection and Drug Resistance·Ehsaneh KhodadadiHossein Samadi Kafil
Nov 10, 2020·Frontiers in Microbiology·Yi-Yun LiuJian-Hua Liu
Nov 17, 2020·Frontiers in Cellular and Infection Microbiology·Ann-Britt Schäfer, Michaela Wenzel
Mar 7, 2021·Microorganisms·Mouna HamelSophie Alexandra Baron
Mar 31, 2021·Critical Reviews in Food Science and Nutrition·Karolinny Cristiny de Oliveira VieiraLizziane Kretli Winkelstroter Eller

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