Comprehensive Proteomics Analysis of Laticifer Latex Reveals New Insights into Ethylene Stimulation of Natural Rubber Production

Scientific Reports
Xuchu WangZheng Tong

Abstract

Ethylene is a stimulant to increase natural rubber latex. After ethylene application, both fresh yield and dry matter of latex are substantially improved. Moreover, we found that ethylene improves the generation of small rubber particles. However, most genes involved in rubber biosynthesis are inhibited by exogenous ethylene. Therefore, we conducted a proteomics analysis of ethylene-stimulated rubber latex, and identified 287 abundant proteins as well as 143 ethylene responsive latex proteins (ERLPs) with mass spectrometry from the 2-DE and DIGE gels, respectively. In addition, more than 1,600 proteins, including 404 ERLPs, were identified by iTRAQ. Functional classification of ERLPs revealed that enzymes involved in post-translational modification, carbohydrate metabolism, hydrolase activity, and kinase activity were overrepresented. Some enzymes for rubber particle aggregation were inhibited to prolong latex flow, and thus finally improved latex production. Phosphoproteomics analysis identified 59 differential phosphoproteins; notably, specific isoforms of rubber elongation factor and small rubber particle protein that were phosphorylated mainly at serine residues. This post-translational modification and isoform-specific pho...Continue Reading

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Citations

Oct 7, 2016·Plant Physiology and Biochemistry : PPB·Zheng TongXuchu Wang
May 30, 2019·International Journal of Molecular Sciences·Quanliang XieXuchu Wang
Dec 26, 2018·International Journal of Molecular Sciences·Xiao MenMo Xian
Apr 26, 2018·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Jin-Ping LiuLiangsheng Zhang
Oct 28, 2020·Journal of Plant Research·Mohd Afiq Hazlami Habib, Mohd Nazri Ismail
Aug 18, 2021·Plant Physiology and Biochemistry : PPB·Longjun DaiLifeng Wang

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Datasets Mentioned

BETA
AJJZ01000000

Methods Mentioned

BETA
electrophoresis
phosphotransferase
aminoacylation
ubiquitination

Software Mentioned

Functional Catalogue
Mascot Algorithm
[UNK]
- Antigen Finder
TargetP
Blast
ImageMaster
Mascot
ProteinPilot
Peptide

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