PMID: 9188614Jul 1, 1997Paper

Comprehensive quantification of herpes simplex virus latency at the single-cell level

Journal of Virology
Nancy M Sawtell

Abstract

To date, characterization of latently infected tissue with respect to the number of cells in the tissue harboring the viral genome and the number of viral genomes contained within individual latently infected cells has not been possible. This level of cellular quantification is a critical step in determining (i) viral or host cell factors which function in the establishment and maintenance of latency, (ii) the relationship between latency burden and reactivation, and (iii) the effectiveness of vaccines or antivirals in reducing or preventing the establishment of latent infections. Presented here is a novel approach for the quantitative analysis of nucleic acids within the individual cells comprising complex solid tissues. One unique feature is that the analysis reflects the nucleic acids within the individual cells as they were in the context of the intact tissue-hence the name CXA, for contextual analysis. Trigeminal ganglia latently infected with herpes simplex virus (HSV) were analyzed by CXA of viral DNA. Both the type and the number of cells harboring the viral genome as well as the number of viral genomes within the individual latently infected cells were determined. Here it is demonstrated that (i) the long-term reposito...Continue Reading

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Citations

Sep 15, 2001·Brain Pathology·R J Cohrs, D H Gilden
Jun 20, 2001·Journal of Virology·R L Thompson, N M Sawtell
Feb 12, 2002·Journal of Virology·Alana M Thackray, Raymond Bujdoso
Jun 21, 2002·The Journal of General Virology·Ker R MarshallChris M Preston
Jan 15, 2003·Clinical Microbiology Reviews·Clinton Jones
Oct 24, 2007·Clinical and Experimental Dermatology·M Fatahzadeh, R A Schwartz
Jun 28, 2008·Seminars in Ophthalmology·Hassanain S TomaJames M Hill
Mar 28, 2009·PLoS Pathogens·Richard L ThompsonNancy M Sawtell
Jan 25, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laura K MackayFrancis R Carbone
Jan 21, 2000·The Journal of General Virology·C M Preston
Feb 20, 2010·Interdisciplinary Perspectives on Infectious Diseases·Guey-Chuen Perng, Clinton Jones
Oct 26, 2010·Photomedicine and Laser Surgery·Hazem Mohammad SalehAdel Khalil Ibrahim
Oct 24, 2012·Journal of Biomedicine & Biotechnology·Jody M WebreHilary W Thompson
Mar 12, 2015·PLoS Pathogens·Chandran RamakrishnaEdouard M Cantin
Feb 7, 2007·Nature Clinical Practice. Neurology·Donald H GildenKenneth L Tyler
Feb 12, 2008·Current Protocols in Molecular Biology·N M Sawtell
Oct 13, 2006·Current Opinion in Infectious Diseases·J D Kriesel

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