Comprehensive Redox Profiling of the Thiol Proteome of Clostridium difficile.

Molecular & Cellular Proteomics : MCP
Susanne SieversKatharina Riedel

Abstract

The strictly anaerobic bacterium C. difficile has become one of the most problematic hospital acquired pathogens and a major burden for health care systems. Although antibiotics work effectively in most C. difficile infections (CDIs), their detrimental effect on the intestinal microbiome paves the way for recurrent episodes of CDI. To develop alternative, non-antibiotics-based treatment strategies, deeper knowledge on the physiology of C. difficile, stress adaptation mechanisms and regulation of virulence factors is mandatory. The focus of this work was to tackle the thiol proteome of C. difficile and its stress-induced alterations, because recent research has reported that the amino acid cysteine plays a central role in the metabolism of this pathogen. We have developed a novel cysteine labeling approach to determine the redox state of protein thiols on a global scale. Applicability of this technique was demonstrated by inducing disulfide stress using the chemical diamide. The method can be transferred to any kind of redox challenge and was applied in this work to assess the effect of bile acids on the thiol proteome of C. difficile We present redox-quantification for more than 1,500 thiol peptides and discuss the general diff...Continue Reading

References

Mar 9, 1978·The New England Journal of Medicine·J G BartlettA B Onderdonk
Feb 15, 2001·Nature Cell Biology·S R JaffreyS H Snyder
Oct 27, 2004·PLoS Biology·Lars I Leichert, Ursula Jakob
Dec 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Adrian T SaurinPhilip Eaton
Oct 1, 2005·Molecular Microbiology·Falko HochgräfeMichael Hecker
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lars I LeichertUrsula Jakob
May 16, 2008·Journal of Medical Microbiology·Jenny E EmersonNeil F Fairweather
Jan 3, 2009·Nature Nanotechnology
Jan 10, 2009·Nature Protocols·Lorenza BordoliTorsten Schwede
Aug 4, 2009·BMC Structural Biology·Bent PetersenClaus Lundegaard
Nov 27, 2009·The Journal of Biological Chemistry·Robert DanielsBirgitta Henriques-Normark
Jun 8, 2010·ACS Chemical Biology·Vaishali SinhaGregory R J Thatcher
Dec 1, 2011·Nucleic Acids Research·Marco PuntaRobert D Finn
Jan 10, 2013·Journal of Proteome Research·Jenn-Wei ChenYung-Fu Chang
Jan 10, 2013·Plant & Cell Physiology·Yu TsukamotoToru Hisabori
Jan 9, 2014·PloS One·Patrick M ChongUNKNOWN Canadian Nosocomial Infection Surveillance Program (CNISP)
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Claudia M CremersUrsula Jakob
May 9, 2014·PharmacoEconomics·Kari A MergenhagenJoseph A Paladino
Sep 10, 2014·The Journal of Biological Chemistry·Samuel MorisseStéphane D Lemaire
Oct 16, 2014·Molecular & Cellular Proteomics : MCP·Jennifer ParkerSixue Chen
Dec 3, 2014·Journal of Proteomics·Katarzyna WojdylaAdelina Rogowska-Wrzesinska
Apr 7, 2015·Free Radical Biology & Medicine·Young-Mi GoDean P Jones
Aug 19, 2015·Redox Biology·Katarzyna Wojdyla, Adelina Rogowska-Wrzesinska
Nov 1, 2015·Molecular & Cellular Proteomics : MCP·Jing YangDaniel C Liebler
Nov 4, 2015·Nucleic Acids Research·Juan Antonio VizcaínoHenning Hermjakob
Jun 15, 2016·Infection and Immunity·Thomas DuboisBruno Dupuy
Jun 29, 2016·Journal of Proteome Research·Kazutaka ArakiTohru Natsume
Aug 12, 2016·Proteomics. Clinical Applications·Andreas OttoSusanne Sievers
Nov 12, 2016·Frontiers in Microbiology·Adrianne N EdwardsShonna M McBride
Feb 12, 2017·Frontiers in Plant Science·Zepeng YinSixue Chen

❮ Previous
Next ❯

Citations

Aug 2, 2019·Antioxidants & Redox Signaling·Jason M Held
Mar 6, 2019·Frontiers in Microbiology·Susanne SieversKatharina Riedel
Dec 26, 2018·Oxidative Medicine and Cellular Longevity·Claudia UrbainskyChristopher Horst Lillig
Mar 30, 2021·Frontiers in Microbiology·Xinrong ZhangXiao-Lin Chen
Oct 28, 2019·Journal of Proteome Research·Atef Mannaa, Franz-Georg Hanisch

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.