Comprehensive spectroscopic, steady state, and transient kinetic studies of a representative siderophore-associated flavin monooxygenase.

The Journal of Biological Chemistry
Jeffery A MayfieldJennifer L DuBois

Abstract

Many siderophores used for the uptake and intracellular storage of essential iron contain hydroxamate chelating groups. Their biosyntheses are typically initiated by hydroxylation of the primary amine side chains of l-ornithine or l-lysine. This reaction is catalyzed by members of a widespread family of FAD-dependent monooxygenases. Here the kinetic mechanism for a representative family member has been extensively characterized by steady state and transient kinetic methods, using heterologously expressed N(5)-l-ornithine monooxygenase from the pathogenic fungus Aspergillus fumigatus. Spectroscopic data and kinetic analyses suggest a model in which a molecule of hydroxylatable substrate serves as an activator for the reaction of the reduced flavin and O(2). The rate acceleration is only ∼5-fold, a mild effect of substrate on formation of the C4a-hydroperoxide that does not influence the overall rate of turnover. The effect is also observed with the bacterial ornithine monooxygenase PvdA. The C4a-hydroperoxide is stabilized in the absence of hydroxylatable substrate by the presence of bound NADP(+) (t(½) = 33 min, 25 °C, pH 8). NADP(+) therefore is a likely regulator of O(2) and substrate reactivity in the siderophore-associated ...Continue Reading

References

Nov 1, 1975·The Biochemical Journal·A C StorerA Cornish-Bowden
Dec 1, 1971·Analytical Biochemistry·G TomlinsonT Viswanatha
Apr 28, 1995·Chemico-biological Interactions·L L Poulsen, D M Ziegler
May 1, 1993·European Journal of Biochemistry·P MacherouxH Diekmann
Nov 14, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·R L Jurado
Apr 9, 1999·Clinical Microbiology Reviews·J P Latgé
Apr 19, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D A StevensG A Pankey
Jun 6, 2000·Journal of Protein Chemistry·S DickT Viswanatha
Aug 22, 2001·Trends in Microbiology·J P Latgé
May 9, 2002·FEBS Letters·Marco W FraaijeDick B Janssen
Jun 1, 2002·Microbiology and Molecular Biology Reviews : MMBR·Jorge H Crosa, Christopher T Walsh
Dec 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Justine P Roth, Judith P Klinman
Jan 7, 2003·Infectious Disease Clinics of North America·Kieren A MarrDavid Denning
Jun 28, 2003·FEMS Microbiology Reviews·Simon C AndrewsFrancisco Rodríguez-Quiñones
Dec 20, 2003·Biochemical and Biophysical Research Communications·Lorenza PutignaniPaolo Visca
Apr 2, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jean-Philippe SalierDarren R Flower
Oct 19, 2004·Annual Review of Microbiology·Cécile Wandersman, Philippe Delepelaire
Oct 27, 2004·The Journal of Experimental Medicine·Markus SchrettlHubertus Haas
Dec 8, 2004·Archives of Biochemistry and Biophysics·Barrie EntschDavid P Ballou
Feb 19, 2005·Chembiochem : a European Journal of Chemical Biology·Gregory L Challis
Jan 6, 2006·Journal of Medicinal Chemistry·Ravindranadh V SomuCourtney C Aldrich
Jan 13, 2006·Annual Review of Pharmacology and Toxicology·John R Cashman, Jun Zhang
Jan 13, 2006·Nature Chemical Biology·Julian A FerrerasLuis E N Quadri
Feb 18, 2006·Nature Chemical Biology·Michael A FischbachChristopher T Walsh
May 16, 2006·Immunobiology·Sek Tong OngJeak Ling Ding
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Michael A FischbachKelly D Smith
Nov 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Rebecca J AbergelKenneth N Raymond
Jun 16, 2007·Organic & Biomolecular Chemistry·Andrew J WalzMarvin J Miller
Feb 19, 2008·Mycological Research·Linda Johnson
Apr 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Andrea AlfieriAndrea Mattevi
Aug 6, 2008·Journal of the American Chemical Society·Rebecca J AbergelKenneth N Raymond
Aug 6, 2008·Annual Review of Phytopathology·Hubertus HaasB Gillian Turgeon
Apr 8, 2009·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Rosanne E FrederickJennifer L DuBois

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Citations

Jul 22, 2011·Journal of the American Chemical Society·Rosanne E FrederickJennifer L DuBois
Nov 29, 2012·The Journal of Biological Chemistry·Xinhua DaiYunde Zhao
Dec 31, 2011·Biochimica Et Biophysica Acta·Stefania Montersino, Willem J H van Berkel
Apr 3, 2012·Biochimica Et Biophysica Acta·Elvira RomeroPablo Sobrado
Jun 24, 2014·The Arabidopsis Book·Yunde Zhao
Feb 19, 2014·Biochimica Et Biophysica Acta·Reeder RobinsonPablo Sobrado
Aug 10, 2016·Archives of Biochemistry and Biophysics·Heba AbdelwahabPablo Sobrado
Oct 6, 2016·PloS One·Heba AbdelwahabPablo Sobrado
Aug 12, 2018·Protein Science : a Publication of the Protein Society·Reeder M RobinsonPablo Sobrado
May 4, 2020·Protein Science : a Publication of the Protein Society·Kuan-Hung LinTsung-Lin Li
Feb 6, 2017·The Journal of Biological Chemistry·Panu PimviriyakulPimchai Chaiyen
Jun 7, 2020·Applied Microbiology and Biotechnology·Carolin MüggeDirk Tischler
Jan 29, 2019·Archives of Biochemistry and Biophysics·Nikola KenjićAudrey L Lamb
Apr 5, 2017·Chemical Reviews·Abraham J WaldmanEmily P Balskus
Jan 13, 2018·Chemical Reviews·Elvira RomeroAndrea Mattevi
Jan 31, 2015·The Journal of Organic Chemistry·Somayesadat BadieyanPablo Sobrado
Sep 14, 2021·Biochemistry·Hannah Valentino, Pablo Sobrado

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