Comprehensive transcription analysis of human pathogenic fungus Penicillium marneffei in mycelial and yeast cells

Medical Mycology
Xinyu LinYaling Dai

Abstract

Penicillium marneffei is a dimorphic fungus that responds to changes in temperature. We performed a comparative analysis of gene expression in mycelial- and yeast-phase P. marneffei cells using high-throughput DNA microarrays. A total of 1,884 differentially expressed genes with annotations in the gene ontology (GO) database were identified between P. marneffei mycelial and yeast cells. These differentially expressed genes mainly belong to 18 categories in the organism's ontology, including reproduction, immunity, metabolism, signaling, etc. Bioinformatics suggests that these differentially expressed genes may help explain the resistance to adverse environments and the virulence of P. marneffei. Nine genes from the results of the DNA microarray experiment were selected for further analysis with real-time quantitative PCR to validate the differential expression. Our data provide a global description of the transcriptional response accompanying adaptation to high temperature in yeast.

References

Aug 1, 1973·American Journal of Clinical Pathology·A F DiSalvoL Ajello
Jul 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·T A Duong
Nov 28, 2002·International Journal of Medical Microbiology : IJMM·Alex Andrianopoulos
Mar 5, 2003·Journal of Cell Science·Kylie J BoyceAlex Andrianopoulos
Mar 18, 2003·Archives of Microbiology·Kwok-Yung YuenAntoine Danchin
Mar 27, 2003·Molecular Microbiology·Richard B ToddAlex Andrianopoulos
Jul 23, 2003·Nature Biotechnology·Junmin PengSteven P Gygi
Apr 9, 2004·Journal of Clinical Microbiology·Brent A Lasker, Yuping Ran
Nov 6, 2004·Journal of Clinical Microbiology·Matthew C FisherNongnuch Vanittanakom
Jan 6, 2005·Microbiology·Sirida YoungchimAndrew J Hamilton
Mar 10, 2005·Eukaryotic Cell·Martine BassilanaRobert A Arkowitz
Jun 21, 2005·Current Biology : CB·Clarissa J Nobile, Aaron P Mitchell
Mar 29, 2006·Journal of Clinical Pathology·D LimA R Chang
Feb 23, 2007·Journal of Medical Microbiology·Liyan XiMengfeng Li
Jan 15, 2008·Current Opinion in Infectious Diseases·Andrew P UstianowskiJeremy N Day
Feb 27, 2008·The Journal of Cell Biology·Hui JinEric Grote
Jun 6, 2008·Proteome Science·Julie M ChandlerChester R Cooper
Feb 24, 2009·Current Opinion in Cell Biology·Jeffrey A Knauf, James A Fagin
Jun 3, 2009·Methods in Molecular Biology·Jonathan R Pollack
Jul 25, 2009·Nature Reviews. Cancer·Erwin F Wagner, Angel R Nebreda
Nov 9, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Thuy LeJeremy N Day

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