Comprehensive Two-dimensional Gas Chromatography Time-of-flight Mass Spectrometry to Assess the Presence of α,α-Trehalose and Other Disaccharides in Apple and Peach

Phytochemical Analysis : PCA
Alexis Marsol-VallRamon Canela-Garayoa

Abstract

Carbohydrates are important constituents in fruits. Among the carbohydrates, disaccharides have rarely been studied in apple and peach. Indeed, the abiotic stress biomarker and preservation agent α,α-trehalose is a disaccharide. To establish a comprehensive method based on two-dimensional gas chromatography combined with time-of-flight MS detection (GC × GC-ToF/MS) to analyse the disaccharide composition of apple and peach. The sample preparation was based on aqueous-methanolic extraction of the analytes, followed by oxime formation and trimethylsilylation of the disaccharides. First, three columns were tested with standards on the one-dimensional system. Next, to perform the sample analysis using GC × GC-MS (which offers significant advantages over conventional GC because it allows higher separation efficiencies), various column configurations were assessed on the two-dimensional system to obtain enhanced separation and low detection limits. The column sets tested included non-polar/semi-polar, semi-polar/polar and polar/non-polar. Using the method that proved to be more efficient, namely the method developed with the semi-polar/non-polar configuration, ten disaccharides were identified, based on analytical standards, retentio...Continue Reading

References

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Citations

Oct 17, 2019·Analytical and Bioanalytical Chemistry·Sonia Rodríguez-SánchezAna I Ruiz-Matute

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