Comprehensively Characterizing the Thioredoxin Interactome In Vivo Highlights the Central Role Played by This Ubiquitous Oxidoreductase in Redox Control

Molecular & Cellular Proteomics : MCP
Isabelle S ArtsJean-François Collet

Abstract

Thioredoxin (Trx) is a ubiquitous oxidoreductase maintaining protein-bound cysteine residues in the reduced thiol state. Here, we combined a well-established method to trap Trx substrates with the power of bacterial genetics to comprehensively characterize the in vivo Trx redox interactome in the model bacterium Escherichia coli Using strains engineered to optimize trapping, we report the identification of a total 268 Trx substrates, including 201 that had never been reported to depend on Trx for reduction. The newly identified Trx substrates are involved in a variety of cellular processes, ranging from energy metabolism to amino acid synthesis and transcription. The interaction between Trx and two of its newly identified substrates, a protein required for the import of most carbohydrates, PtsI, and the bacterial actin homolog MreB was studied in detail. We provide direct evidence that PtsI and MreB contain cysteine residues that are susceptible to oxidation and that participate in the formation of an intermolecular disulfide with Trx. By considerably expanding the number of Trx targets, our work highlights the role played by this major oxidoreductase in a variety of cellular processes. Moreover, as the dependence on Trx for re...Continue Reading

References

Apr 20, 2017·Nature Reviews. Microbiology·B EzratyJean-François Collet
Dec 13, 2019·Expert Review of Proteomics·David Sheehan, Brian McDonagh
Jan 26, 2018·The Journal of Biological Chemistry·Camille V GoemansJean-François Collet

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Related Concepts

In Vivo
Carbohydrate nutrients
Cellular Process
Alkalescens-Dispar Group
Phosphoenolpyruvate-Glycose Phosphotransferase System
Thioredoxin-2
Actins
Energy Metabolism
Transcription, Genetic
Sulfhydryl Compounds

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