Compromised Barrier Function in Human Induced Pluripotent Stem-Cell-Derived Retinal Pigment Epithelial Cells from Type 2 Diabetic Patients

International Journal of Molecular Sciences
Mostafa KiamehrK Juuti-Uusitalo

Abstract

In diabetic patients, high blood glucose induces alterations in retinal function and can lead to visual impairment due to diabetic retinopathy. In immortalized retinal pigment epithelial (RPE) cultures, high glucose concentrations are shown to lead to impairment in epithelial barrier properties. For the first time, the induced pluripotent stem-cell-derived retinal pigment epithelium (hiPSC-RPE) cell lines derived from type 2 diabetics and healthy control patients were utilized to assess the effects of glucose concentration on the cellular functionality. We show that both type 2 diabetic and healthy control hiPSC-RPE lines differentiate and mature well, both in high and normal glucose concentrations, express RPE specific genes, secrete pigment epithelium derived factor, and form a polarized cell layer. Here, type 2 diabetic hiPSC-RPE cells had a decreased barrier function compared to controls. Added insulin increased the epithelial cell layer tightness in normal glucose concentrations, and the effect was more evident in type 2 diabetics than in healthy control hiPSC-RPE cells. In addition, the preliminary functionality assessments showed that type 2 diabetic hiPSC-RPE cells had attenuated autophagy detected via ubiquitin-binding...Continue Reading

References

Dec 15, 1992·American Journal of Ophthalmology·A M Abu el AsrarA T Reder
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 16, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Stephen J GiebelArup Das
Dec 15, 2007·Nature Protocols·Kazutoshi TakahashiShinya Yamanaka
Jun 19, 2009·Current Protocols in Stem Cell Biology·Mari OhnukiShinya Yamanaka
Dec 24, 2010·Investigative Ophthalmology & Visual Science·Hui-Zhuo Xu, Yun-Zheng Le
Aug 11, 2012·Stem Cell Research·Riikka J LundRiitta Lahesmaa
Sep 13, 2012·Current Eye Research·Karunakaran CoralNarayanasamy Angayarkanni
Feb 19, 2013·Progress in Retinal and Eye Research·Ingeborg KlaassenReinier O Schlingemann
Oct 10, 2013·Progress in Retinal and Eye Research·S BorooahB Dhillon
Feb 25, 2014·ISRN Ophthalmology·Joanna M TarrRakesh Chibber
Aug 15, 2014·BioMed Research International·Selina BeasleyMohamed Al-Shabrawey
Feb 18, 2015·BioMed Research International·Andrea MatteucciFiorella Malchiodi-Albedi
Aug 25, 2015·Progress in Retinal and Eye Research·Alan W StittNoemi Lois
Mar 22, 2016·Current Neuropharmacology·Michelino Di RosaLucia Malaguarnera
Jul 22, 2016·American Journal of Physiology. Cell Physiology·Mitra FarnoodianNader Sheibani
Aug 4, 2016·Journal of Cellular Physiology·Grazia MaugeriVelia D'Agata
Jan 21, 2017·Journal of Cellular Biochemistry·Grazia MaugeriVelia D'Agata
Mar 11, 2017·Cytokine·Murugeswari PonnalaguDebashish Das
May 26, 2017·Cellular & Molecular Biology Letters·Wei Jing LiuHua Feng Liu
Jul 12, 2017·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Grazia MaugeriVelia D'Agata
Jul 25, 2017·Disease Models & Mechanisms·Mostafa KiamehrKatriina Aalto-Setälä
Apr 27, 2018·Advanced Drug Delivery Reviews·Kai KaarnirantaAnu Kauppinen
Jan 15, 2019·Archives of Biochemistry and Biophysics·Shawn M Sternisha, Brian G Miller
Mar 8, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yuanping ZhangYanni Yang

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Citations

Jan 21, 2021·Nature Reviews. Endocrinology·David A AntonettiAlan W Stitt
Jul 30, 2020·Progress in Retinal and Eye Research·Sven SchnichelsAlexa Klettner
Dec 22, 2020·Journal of Neuroscience Methods·Laura HonkamäkiSusanna Narkilahti
Jul 9, 2021·Frontiers in Pharmacology·Annagrazia AdornettoRossella Russo

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Methods Mentioned

BETA
PCR
transmission electron microscopy
biopsy
Enzyme-Linked Immunosorbent Assay

Software Mentioned

7300 System SDS
PASW Statistics

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