Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic Rats.

Digestive Diseases and Sciences
Linhao ZhangChengwei Tang

Abstract

In liver cirrhosis, intestinal mucus barrier is rarely studied. This study aimed to investigate whether mucus barrier in ileum is altered in cirrhotic rats and its underlying mechanisms. Thioacetamide was injected to induce liver cirrhosis in rats. Serum from portal vein blood, and ileum and liver tissues were obtained for further analysis. Goblet cell-like Ls174T cells were cultured for in vitro experiments. The ileal mucus was thin, loose, and porous with small bubbles in cirrhotic rats. mRNA expressions of Muc2 and TFF3 were also down-regulated in cirrhotic rats. Bacteria located near to crypts and LPS were increased in the serum from portal vein in cirrhotic rats. Smaller theca area and few goblet cells were found in cirrhotic rats compared with control. Increased proliferation of ileal epithelia was observed in cirrhotic rats. Notch1, Dll1, and Hes1 expressions were enhanced, and KLF4 expression was suppressed in ileum of cirrhotic rats. In Ls174T cells, EDTA and NICD plasmid induced NICD and Hes1 expression and suppressed KLF4 concomitantly, and mucus expression almost vanished in these cells. NICD plasmid induced more proliferation in Ls174T cells. Oppositely, after DBZ treatment, NICD and Hes1 were inhibited along with ...Continue Reading

References

Jan 16, 1998·Journal of Gastroenterology·H ImanishiM Makuuchi
Feb 12, 2000·Molecular and Cellular Biology·M D RandJ C Aster
Jan 27, 2005·Biochemical and Biophysical Research Communications·Katsumasa SuzukiTsutomu Chiba
Jun 17, 2005·Nature·Silvia FreSpyros Artavanis-Tsakonas
Jul 13, 2006·Gastroenterology·Maria Van der SluisAlexandra W C Einerhand
Jul 7, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Robert C De LisleKyle Jansson
Dec 17, 2008·Molecular Cancer Research : MCR·Amr M GhalebVincent W Yang
Dec 26, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hai ZhengWalden Ai
Apr 22, 2009·Cell·Raphael Kopan, Maria Xenia G Ilagan
Oct 27, 2009·Nature Reviews. Immunology·Jerrold R Turner
Feb 9, 2010·Gastroenterology·Sumaira Z HasnainWaliul I Khan
Apr 19, 2013·Gut·Michael A McGuckin, Sumaira Z Hasnain
Apr 8, 2015·World Journal of Hepatology·Hiroshi Fukui
Jul 7, 2015·Trends in Molecular Medicine·Heather A McCauley, Géraldine Guasch
Oct 28, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Aiping WangFayez K Ghishan
Feb 13, 2016·Virulence·Michele BartolettiPierluigi Viale
Aug 9, 2016·Nature Reviews. Immunology·Malin E V Johansson, Gunnar C Hansson
May 21, 2017·Journal of Hepatology·Reiner WiestRajiv Jalan

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Metabolism: Clinical and Experimental
T NakamuraS Baba
La Revue du praticien
C BéraudP Faucher
Revue médico-chirurgicale des maladies du foie
P MagnenatJ P Felber
Médecine & chirurgie digestives
P E MorganteD Kravetz
© 2021 Meta ULC. All rights reserved