Computational identification of putative miRNAs and their target genes in pathogenic amoeba Naegleria fowleri

Bioinformation
Dyavegowda Padmashree, Narayanaswamy Ramachandra Swamy

Abstract

Naegleria fowleri is a parasitic unicellular free living eukaryotic amoeba. The parasite spreads through contaminated water and causes primary amoebic meningoencephalitis (PAM). Therefore, it is of interest to understand its molecular pathogenesis. Hence, we analyzed the parasite genome for miRNAs (microRNAs) that are non-coding, single stranded RNA molecules. We identified 245 miRNAs using computational methods in N. fowleri, of which five miRNAs are conserved. The predicted miRNA targets were analyzed by using miRanda (software) and further studied the functions by subsequently annotating using AmiGo (a gene ontology web tool).

Citations

Mar 31, 2015·ELife·Gareth BloomfieldRobert R Kay
Jul 24, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Vahid RaissiAsmaa Ibrahim

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Software Mentioned

AmiGo
BlastX
RNA
Java
MatureBayes
RNA fold
BLAST
Einverted
fold
BlastN

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