Computational Image Analysis Reveals Intrinsic Multigenerational Differences between Anterior and Posterior Cerebral Cortex Neural Progenitor Cells

Stem Cell Reports
Mark WinterAndrew R Cohen

Abstract

Time-lapse microscopy can capture patterns of development through multiple divisions for an entire clone of proliferating cells. Images are taken every few minutes over many days, generating data too vast to process completely by hand. Computational analysis of this data can benefit from occasional human guidance. Here we combine improved automated algorithms with minimized human validation to produce fully corrected segmentation, tracking, and lineaging results with dramatic reduction in effort. A web-based viewer provides access to data and results. The improved approach allows efficient analysis of large numbers of clones. Using this method, we studied populations of progenitor cells derived from the anterior and posterior embryonic mouse cerebral cortex, each growing in a standardized culture environment. Progenitors from the anterior cortex were smaller, less motile, and produced smaller clones compared to those from the posterior cortex, demonstrating cell-intrinsic differences that may contribute to the areal organization of the cerebral cortex.

References

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Mar 23, 2012·International Journal of Computational Biology and Drug Design·Mark R WinterAndrew R Cohen
Jan 21, 2014·Nature Methods·Nicolas ChenouardErik Meijering
Apr 9, 2014·IEEE Transactions on Medical Imaging·Xinghua LouFred A Hamprecht

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Citations

Mar 26, 2016·Frontiers in Neuroscience·Felipe Ortega, Marcos R Costa
Dec 20, 2016·Nature Communications·M Cecilia CainoDario C Altieri
Nov 20, 2016·Annual Review of Physiology·Elizabeth A SpechtAmy E Palmer
Feb 22, 2017·Nature Methods·Felix BuggenthinCarsten Marr
Mar 16, 2017·Immunology and Cell Biology·Andrey Kan
May 19, 2016·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Rohini JoshiAndrew R Cohen
Jun 19, 2018·APL Bioengineering·Kamyar Esmaeili PourfarhangiBojana Gligorijevic
Jun 15, 2018·Cancer Research·Jae Ho SeoDario C Altieri
Feb 23, 2021·PLoS Computational Biology·Mark R WinterSmadar Ben-Tabou de-Leon
Dec 2, 2020·Current Opinion in Neurobiology·Evelyne Fischer, Xavier Morin
May 11, 2020·Cell Stem Cell·Kara L McKinleyOphir D Klein

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Methods Mentioned

BETA
flow cytometry
FACS
fluorescence imaging

Software Mentioned

MAT
LEVER
MATLAB
Lineage Editing and Validation ( LEVER )
CloneView
Windows

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