Computational imaging modalities for multi-focal whole-slide imaging systems.

Applied Optics
Leon van der GraaffSjoerd Stallinga

Abstract

Whole-slide imaging systems can generate full-color image data of tissue slides efficiently, which are needed for digital pathology applications. This paper focuses on a scanner architecture that is based on a multi-line image sensor that is tilted with respect to the optical axis, such that every line of the sensor scans the tissue slide at a different focus level. This scanner platform is designed for imaging with continuous autofocus and inherent color registration at a throughput of the order of 400 MPx/s. Here, single-scan multi-focal whole-slide imaging, enabled by this platform, is explored. In particular, two computational imaging modalities based on multi-focal image data are studied. First, 3D imaging of thick absorption stained slides (∼60µm) is demonstrated in combination with deconvolution to ameliorate the inherently weak contrast in thick-tissue imaging. Second, quantitative phase tomography is demonstrated on unstained tissue slides and on fluorescently stained slides, revealing morphological features complementary to features made visible with conventional absorption or fluorescence stains. For both computational approaches simplified algorithms are proposed, targeted for straightforward parallel processing imp...Continue Reading

References

Aug 6, 2005·Advances in Biochemical Engineering/biotechnology·Jean-Baptiste Sibarita
Apr 29, 2006·BMC Clinical Pathology·John R GilbertsonAnil V Parwani
Aug 18, 2006·Micron : the International Research and Review Journal for Microscopy·Rainer Heintzmann
Oct 17, 2006·International Journal of Surgical Pathology·Marcial García RojoManuel Carbajo Vicente
Jul 7, 2007·Journal of Biomedical Optics·Miguel E Bravo-ZanogueraJeffrey H Price
Apr 9, 2008·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·C Vonesch, M Unser
Jul 17, 2008·American Journal of Clinical Pathology·Thomas KalinskiAlbert Roessner
Jul 3, 2010·Optics Letters·Jigang WuChanghuei Yang
Oct 6, 2010·Journal of Pathology Informatics·Kirk Foster
Mar 4, 2011·Optics Express·Laura WallerGeorge Barbastathis
Jun 7, 2011·Journal of Biomedical Optics·Sanjee AbeytungeMilind Rajadhyaksha
Jul 26, 2011·Pathology Research International·Walid E KhalbussAnil V Parwani
Mar 21, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Peter W HamiltonStephen J McCullough
Apr 12, 2012·The American Journal of Pathology·Nicholas RobertsDarren Treanor
Apr 14, 2012·Advances in Anatomic Pathology·Toby C CornishKeith J Kaplan
Nov 20, 2012·Annual Review of Pathology·Farzad GhaznaviMichael Feldman
Dec 4, 2012·Optics Letters·Shuo PangChanghuei Yang
May 3, 2013·Archives of Pathology & Laboratory Medicine·Liron PantanowitzUNKNOWN College of American Pathologists Pathology and Laboratory Quality Center
Jul 19, 2014·Methods : a Companion to Methods in Enzymology·Peter W HamiltonManuel Salto-Tellez
Sep 23, 2014·Nature Photonics·Guoan ZhengChanghuei Yang
Apr 4, 2015·Optics Express·S Mojtaba ShakeriSjoerd Stallinga
Sep 27, 2015·Histopathology·David R J SneadIan A Cree
Dec 20, 2015·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Emrah BostanMichael Unser
May 5, 2016·Progress in Histochemistry and Cytochemistry·Adriano AzaripourCornelis J F Van Noorden
Jun 7, 2017·Journal of Pathology Informatics·Esther Abels, Liron Pantanowitz
Jul 25, 2017·Optica·Roarke HorstmeyerChanghuei Yang
Dec 20, 2019·Biomedical Optics Express·Leon van der GraaffSjoerd Stallinga

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.