Computational Modeling and Clonogenic Assay for Radioenhancement of Gold Nanoparticles Using 3D Live Cell Images

Radiation Research
Wonmo SungSung-Joon Ye

Abstract

Radioenhancement of gold nanoparticles (GNPs) has shown great potential for increasing the therapeutic efficiency of radiotherapy. Here we report on a computational model of radiation response, which was developed to predict the survival curves of breast cancer cells incubated with GNPs. The amount of GNP uptake was estimated using inductively coupled plasma-mass spectroscopy, and the three-dimensional (3D) intracellular distribution of GNPs was obtained using optical diffraction tomography. The developed computational model utilized the 3D live cell imaging and recent Monte Carlo techniques to calculate microscopic dose distributions within the cell. Clonogenic assays with and without GNPs were performed to estimate the radioenhancement for 150 kVp X rays in terms of cell survival fractions. Measured cell survival fractions were comparable with the computational model.

References

Dec 1, 2000·Physics in Medicine and Biology·M KrämerU Weber
Oct 29, 2004·Physics in Medicine and Biology·James F HainfeldHenry M Smilowitz
Mar 31, 2009·Toxicology and Applied Pharmacology·Yan-Juan GuWing-Tak Wong
Mar 31, 2009·Colloids and Surfaces. B, Biointerfaces·Omer Faruk KarataşMustafa Culha
May 30, 2009·Nanomedicine : Nanotechnology, Biology, and Medicine·Wan Nordiana RahmanMoshi Geso
Sep 3, 2009·Physics in Medicine and Biology·G PoludniowskiF Verhaegen
May 14, 2010·Physics in Medicine and Biology·James F HainfeldHenry M Smilowitz
Nov 26, 2010·International Journal of Radiation Oncology, Biology, Physics·Suneil JainDavid G Hirst
Oct 20, 2011·Molecular BioSystems·Yiyan FeiXiangdong Zhu
Feb 23, 2012·Scientific Reports·Stephen J McMahonFred J Currell
Jun 16, 2012·International Journal of Nanomedicine·Jonathan A CoulterKevin M Prise
May 9, 2015·Physics in Medicine and Biology·Yuting LinJan Schuemann
Sep 30, 2015·Nano Letters·Sijumon KunjachanRoss I Berbeco
Dec 25, 2015·International Journal of Radiation Oncology, Biology, Physics·Jan SchuemannSunil Krishnan
Jan 21, 2016·Scientific Reports·Harold N McQuaidFred J Currell
Feb 20, 2016·Journal of Biomedical Optics·Hyeonseung YuYongKeun Park
Jul 21, 2016·Physics in Medicine and Biology·A L McNamaraZ Kuncic
Oct 21, 2016·Physics in Medicine and Biology·Wonmo SungSung-Joon Ye
Dec 27, 2016·Physica Medica : PM : an International Journal Devoted to the Applications of Physics to Medicine and Biology : Official Journal of the Italian Association of Biomedical Physics (AIFB)·Aimee McNamaraJan Schuemann
Apr 22, 2017·Nanoscale·Wonmo SungJan Schuemann
Jul 21, 2017·Methods : a Companion to Methods in Enzymology·Doyeon KimYongKeun Park

❮ Previous
Next ❯

Methods Mentioned

BETA
imaging techniques
confocal microscopy
optical diffraction
X-ray
irradiate
Assay
fluorescence imaging

Software Mentioned

SpekCalc
GNP
nBio
TOol for PArticle Simulation ( TOPAS
TomoStudio
ODT
MATLAB
TOPAS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.