Computational modeling and fluorescence microscopy characterization of a two-phase magnetophoretic microsystem for continuous-flow blood detoxification

Lab on a Chip
Jenifer Gómez-PastoraInmaculada Ortiz

Abstract

Magnetic beads can be functionalized to capture and separate target pathogens from blood for extracorporeal detoxification. The beads can be magnetically separated from a blood stream and collected into a coflowing buffer solution using a two-phase liquid-liquid continuous-flow microfluidic device in the presence of an external field. However, device design and process optimization, i.e. high bead recovery with minimum blood loss or dilution remain a substantial technological challenge. We introduce a CFD-based Eulerian-Lagrangian computational model that enables the rational design and optimization of such systems. The model takes into account dominant magnetic and hydrodynamic forces on the beads as well as coupled bead-fluid interactions. Fluid flow (Navier-Stokes equations) and mass transfer (Fick's law) between the coflowing fluids are solved numerically, while the magnetic force on the beads is predicted using analytical methods. The model is demonstrated via application to a prototype device and used to predict key performance metrics; degree of bead separation, flow patterns, and mass transfer, i.e. blood diffusion to the buffer phase. The impact of different process variables and parameters - flow rates, bead and magne...Continue Reading

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Citations

Jul 14, 2020·Separation and Purification Technology·Jenifer Gómez-PastoraJeffrey J Chalmers
Dec 7, 2021·Industrial & Engineering Chemistry Research·Cristina González-FernándezInmaculada Ortiz
Jan 15, 2022·Analytical Chemistry·Hamid AghamohammadiMahla Poudineh

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Methods Mentioned

BETA
fluorescence microscopy
chip
blood draw
rheology

Software Mentioned

- 3D
ProgRes® CapturePro
Flow
MatLab
CapturePro
Visual Studio
3D

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