Computational prediction of furin cleavage sites by a hybrid method and understanding mechanism underlying diseases.

Scientific Reports
Sun TianJianhua Wu

Abstract

Furin cleaves diverse types of protein precursors in the secretory pathway. The substrates for furin cleavage possess a specific 20-residue recognition sequence motif. In this report, based on the functional characterisation of the 20-residue sequence motif, we developed a furin cleavage site prediction tool, PiTou, using a hybrid method composed of a hidden Markov model and biological knowledge-based cumulative probability score functions. PiTou can accurately predict the presence and location of furin cleavage sites in protein sequences with high sensitivity (96.9%) and high specificity (97.3%). PiTou's prediction scores are biological meaningful and reflect binding strength and solvent accessibility of furin substrates. A prediction result is interpreted within cellular contexts: subcellular localisation, cellular function and interference by other dynamic protein modifications. Combining next-generation sequencing, PiTou can help with elucidating the molecular mechanism of furin cleavage-associated human diseases. PiTou has been made freely available at the associated website.

References

Oct 1, 1988·International Journal of Peptide and Protein Research·J L FauchèreV Pliska
Apr 2, 1974·Archives of Biochemistry and Biophysics·H B Bull, K Breese
Nov 1, 1968·Journal of Theoretical Biology·J M ZimmermanR Simha
Jan 1, 1984·Annual Review of Biochemistry·D Eisenberg
Dec 10, 1998·Nucleic Acids Research·S KawashimaM Kanehisa
May 30, 2001·European Journal of Human Genetics : EJHG·M C VincentP Calvas
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y ChenN P Morris
Oct 3, 2002·Nature Reviews. Molecular Cell Biology·Gary Thomas
Apr 23, 2003·Journal of Molecular Biology·Georg NeubergerFrank Eisenhaber
May 9, 2003·The Journal of Biological Chemistry·Chengliang WuQingyu Wu
Feb 27, 2004·Protein Engineering, Design & Selection : PEDS·Peter DuckertNikolaj Blom
Nov 16, 2004·Neural Networks : the Official Journal of the International Neural Network Society·Gavin C Cawley, Nicola L C Talbot
Feb 28, 2009·Clinical Chemistry·Karl V VoelkerdingJacob D Durtschi
Dec 3, 2009·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Christelle Clément-Duchêne, Heather Wakelee
May 11, 2010·PloS One·Katarina HajdinMichele Bernasconi
May 5, 2011·International Journal of Molecular Sciences·Sun TianJianhua Wu

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Citations

Dec 3, 2014·Virus Research·Jean Kaoru Millet, Gary R Whittaker
Oct 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jean Kaoru Millet, Gary R Whittaker
Apr 5, 2017·The Journal of Cell Biology·Istvan FoldiAlicia Hidalgo
Mar 28, 2018·Scientific Reports·Mariko YamadaNorio Takagi
Nov 22, 2013·Journal of Virology·Longping V TseGary R Whittaker
Dec 22, 2016·Emerging Microbes & Infections·Jean Kaoru MilletGary R Whittaker
Jul 18, 2019·Human Vaccines & Immunotherapeutics·Jedhan Ucat GalulaDay-Yu Chao
Feb 14, 2019·Virus Genes·Katarzyna Domańska-BlicharzJoanna Sajewicz-Krukowska
Apr 25, 2020·EMBO Reports·Yiping YuYing-Pu Sun
May 6, 2020·PloS One·Karin LöwAlexandre Roulin
Jan 5, 2013·The Journal of Biological Chemistry·Eva C EnnemannThomas Dierks
Oct 31, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna OksanenMarko Pesu
Oct 25, 2020·The Protein Journal·Mihaela Ileana Ionescu
Apr 17, 2021·Proceedings of the National Academy of Sciences of the United States of America·Kaitlin SawatzkiJonathan A Runstadler
Aug 9, 2021·Current Opinion in Virology·Christian S StevensBenhur Lee

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Methods Mentioned

BETA
glycosylation

Software Mentioned

ProP
PiTou

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