Computational screening and characterization of putative vaccine candidates of Plasmodium vivax

Journal of Biomolecular Structure & Dynamics
Y Nanda KumarPurushothaman Jambulingam

Abstract

Plasmodium vivax is the most prevalent species of malaria affecting millions of people annually worldwide and demands effective interventions to develop a successful vaccine. In this milieu, we have dedicated noteworthy efforts to characterize the proteome of P. vivax to give a lead for the epitope-based vaccine development. Membrane proteins of P. vivax were collected from SWISS PROT database and 10 antigenic proteins were identified among them by in silico analysis using multiple servers. T-cell and B-cell epitopes were identified and their immunity was assessed. Their ability to trigger humoral and cell-mediated responses was determined. Three dimensional models were constructed for the antigenic proteins using Modeller, Phyre2, and Modloop tools and their quality was validated using PROCHECK and ProSA-web validation servers. Further, the binding affinity and molecular interactions of these antigenic proteins were characterized by performing protein-protein docking against transmission-blocking anti-malaria antibody Fab2A8 (PDB ID: 3S62) using Z-dock module of Discovery Studio 4.0. The presence of potential B & T-cell epitopes, major histocompatibility complex-binding sites, and their efficient interactions with Fab2A8 antib...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Aug 7, 1999·Revista Do Instituto De Medicina Tropical De São Paulo·L I MoncadaA Corredor
Jul 13, 2000·Journal of Molecular Biology·O EmanuelssonG von Heijne
Jun 27, 2001·The American Journal of Tropical Medicine and Hygiene·K MendisR Carter
Feb 20, 2002·Biologicals : Journal of the International Association of Biological Standardization·A SetteR Chesnut
Apr 4, 2002·The Journal of Infectious Diseases·Stephen L HoffmanThomas L Richie
Sep 13, 2002·BMC Bioinformatics·Pierre Dönnes, Arne Elofsson
Jun 26, 2003·Nucleic Acids Research·Narayanan EswarAndrej Sali
Jun 26, 2003·Nucleic Acids Research·Pingping GuanDarren R Flower
Jul 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Denise L DoolanAlessandro Sette
Sep 25, 2003·The Journal of Experimental Biology·D L DoolanD J Carucci
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
Jul 16, 2004·Journal of Virology·Bimmi Shrestha, Michael S Diamond
Jul 20, 2004·Annals of Tropical Medicine and Parasitology·M A KurcerS Topluoglu
Aug 7, 2004·Vaccine·Manoj Bhasin, G P S Raghava
Oct 27, 2004·Antimicrobial Agents and Chemotherapy·J Kevin Baird
Jun 27, 2006·Trends in Parasitology·Carlos A GuerraSimon I Hay
Jul 18, 2006·Nucleic Acids Research·Sudipto Saha, G P S Raghava
Nov 15, 2006·Journal of Clinical Pharmacology·Johanna P Daily
Nov 16, 2006·BMC Bioinformatics·Jesper Salomon, Darren R Flower
Jan 9, 2007·BMC Bioinformatics·Irini A Doytchinova, Darren R Flower
Apr 17, 2007·Cellular Immunology·Asif M KhanJ Thomas August
May 23, 2007·Nucleic Acids Research·Markus Wiederstein, Manfred J Sippl
Dec 20, 2007·Immunogenetics·Oleksiy KarpenkoYang Dai
Dec 21, 2007·PLoS Medicine·James G Beeson, Brendan S Crabb
Feb 25, 2009·Bioinformation·Satarudra Prakash Singh, Bhartendu Nath Mishra
Feb 28, 2009·Nature Protocols·Lawrence A Kelley, Michael J E Sternberg
Jul 31, 2009·The New England Journal of Medicine·Meta RoestenbergRobert Sauerwein
Dec 17, 2009·Human Vaccines·Myriam Arévalo-HerreraSócrates Herrera
Nov 1, 2011·International Journal of Biological Macromolecules·Ajay K Saxena
Feb 22, 2012·Biotechnology Advances·Jan BrezovskyJiri Damborsky
May 19, 2012·Seminars in Oncology·Jay A BerzofskyLauren V Wood

❮ Previous
Next ❯

Citations

Feb 10, 2016·Frontiers in Immunology·Josué da Costa Lima-Junior, Lilian Rose Pratt-Riccio
Sep 27, 2018·Frontiers in Genetics·Paola Andrea Camargo-AyalaManuel A Patarroyo

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray

Software Mentioned

align
PLS
SVMHC
IEDB
SMM
MetaMHCII
ProSA
MetaSVMp
BLAST
ProtParam

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.