Computer-aided assessment of the extra-cellular matrix during pancreatic carcinogenesis: a pilot study

Journal of Translational Medicine
Fabio GrizziLuca Di Tommaso

Abstract

A hallmark of pancreatic ductal adenocarcinoma is the desmoplastic reaction, but its impact on the tumor behavior remains controversial. Our aim was to introduce a computer -aided method to precisely quantify the amount of pancreatic collagenic extra-cellular matrix, its spatial distribution pattern, and the degradation process. A series of normal, inflammatory and neoplastic pancreatic ductal adenocarcinoma formalin-fixed and paraffin-embedded Sirius red stained sections were automatically digitized and analyzed using a computer-aided method. We found a progressive increase of pancreatic collagenic extra-cellular matrix from normal to the inflammatory and pancreatic ductal adenocarcinoma. The two-dimensional fractal dimension showed a significant difference in the collagenic extra-cellular matrix spatial complexity between normal versus inflammatory and pancreatic ductal adenocarcinoma. A significant difference when comparing the number of cycles necessary to degrade the pancreatic collagenic extra-cellular matrix in normal versus inflammatory and pancreatic ductal adenocarcinoma was also found. The difference between inflammatory and pancreatic ductal adenocarcinoma was also significant. Furthermore, the mean velocity of coll...Continue Reading

References

Mar 20, 2002·Nature Reviews. Cancer·M J Bissell, D Radisky
Dec 3, 2002·Nature Reviews. Cancer·Nabeel Bardeesy, Ronald A DePinho
Jan 11, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·James N WeissAlan Garfinkel
Sep 20, 2005·Cancer Cell·Matthew J PaszekValerie M Weaver
Apr 13, 2006·World Journal of Gastroenterology : WJG·Nicola DioguardiCarlo Russo
Dec 16, 2006·World Journal of Gastroenterology : WJG·Fabio GrizziNicola Dioguardi
Nov 28, 2007·Annual Review of Pathology·Anirban Maitra, Ralph H Hruban
Apr 15, 2008·Progress in Biophysics and Molecular Biology·Donald E Ingber
Jul 22, 2008·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Mert ErkanJörg Kleeff
Dec 26, 2008·World Journal of Gastroenterology : WJG·Nicola DioguardiCarlo Russo
Apr 30, 2010·The New England Journal of Medicine·Manuel Hidalgo
Oct 23, 2010·Gut·Albrecht NeesseDavid A Tuveson
Jan 10, 2012·Gastroenterology·Mitsuro KandaMichael Goggins
Aug 31, 2012·Proceedings of the National Academy of Sciences of the United States of America·Triantafyllos StylianopoulosRakesh K Jain
Jul 17, 2013·CA: a Cancer Journal for Clinicians·Christopher L WolfgangRalph H Hruban
Jul 31, 2013·Current Opinion in Gastroenterology·Meghna WaghrayDiane M Simeone
Oct 22, 2013·Cancer Letters·Serena LunardiThomas B Brunner
Jan 22, 2014·The Cancer Journal·Robert W Cowan, Anirban Maitra
Jan 24, 2014·Molecular Cancer Research : MCR·Fayth L Miles, Robert A Sikes
Mar 8, 2014·World Journal of Gastroenterology : WJG·Agnieszka Anna Rucki, Lei Zheng
May 21, 2014·Cancer Biology & Therapy·Joji Kurian ThomasAkhilesh Pandey
Jun 11, 2014·Nature Reviews. Gastroenterology & Hepatology·Isobel Leake
Jun 18, 2014·Cancer Cell·Jesse Gore, Murray Korc
Aug 26, 2014·World Journal of Gastroenterology : WJG·Cristiana Pistol TanaseRadu Albulescu
Sep 25, 2014·HPB : the Official Journal of the International Hepato Pancreato Biliary Association·Katherine M BeverLei Zheng
Oct 19, 2014·Journal of the National Cancer Institute·Esther HermanoMichael Elkin
Jan 7, 2015·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Feb 20, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Clifford J WhatcottHaiyong Han
Mar 3, 2015·Seminars in Oncology·Geoffrey KozakJonathan R Brody
Apr 29, 2015·Organogenesis·Andrew M HandorfWan-Ju Li
May 23, 2015·Gut·Albrecht NeesseThomas M Gress
Jul 5, 2015·Journal of the National Cancer Institute·Vicki Brower
Jul 21, 2015·Clinics and Research in Hepatology and Gastroenterology·Atsushi Masamune, Tooru Shimosegawa
Sep 5, 2015·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Cole R DrifkaW John Kao
Mar 15, 2016·World Journal of Gastroenterology : WJG·Michael Friberg Bruun NielsenSönke Detlefsen

❮ Previous
Next ❯

Citations

Jan 24, 2021·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Xintong JiaXudong Zhao

❮ Previous
Next ❯

Methods Mentioned

BETA
surgical resection

Software Mentioned

Statistica
GraphPad Prism
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.