Computer-aided search for a cold-active cellobiose 2-epimerase

Journal of Dairy Science
Qiuming ChenWanmeng Mu

Abstract

Cellobiose 2-epimerase (CE) is a promising industrial enzyme that can catalyze bioconversion of lactose to its high-value derivatives, namely epilactose and lactulose. A need exists in the dairy industry to catalyze lactose bioconversions at low temperatures to avoid microbial growth. We focused on the discovery of cold-active CE in this study. A genome mining method based on computational prediction was used to screen the potential genes encoding cold-active enzymes. The CE-encoding gene from Roseburia intestinalis, with a predicted high structural flexibility, was expressed heterologously in Escherichia coli. The catalytic property of the recombinant enzyme was extensively studied. The optimum temperature and pH of the enzyme were 45°C and 7.0, respectively. The specific activity of this enzyme to catalyze conversion of lactose to epilactose was measured to be 77.3 ± 1.6 U/mg. The kinetic parameters, including turnover number (kcat), Michaelis constant (Km), and catalytic efficiency (kcat/Km) using lactose as a substrate were 117.0 ± 7.7 s-1, 429.9 ± 57.3 mM, and 0.27 mM-1s-1, respectively. In situ production of epilactose was carried out at 8°C: 20.9% of 68.4 g/L lactose was converted into epilactose in 4 h using 0.02 mg/mL ...Continue Reading

References

Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne
Jan 4, 2003·The Journal of Biological Chemistry·Salvino D'AmicoGeorges Feller
Mar 24, 2004·Nature Reviews. Microbiology·Georges Feller, Charles Gerday
Jul 7, 2007·Biochemical and Biophysical Research Communications·Shigeaki ItoSusumu Ito
Nov 29, 2008·Journal of Dairy Science·J WatanabeS Ito
Feb 10, 2009·Bioscience, Biotechnology, and Biochemistry·Takeshi SenouraSusumu Ito
Oct 24, 2009·Current Opinion in Biotechnology·Taku Uchiyama, Kentaro Miyazaki
May 3, 2011·European Biophysics Journal : EBJ·Nathan SchmidWilfred F van Gunsteren
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Jul 27, 2012·Current Opinion in Microbiology·Ben Temperton, Stephen J Giovannoni
Jan 8, 2013·Bioscience, Biotechnology, and Biochemistry·Teruyo OjimaHirokazu Matsui
Jun 27, 2013·Applied Microbiology and Biotechnology·Jin-Song GongZheng-Hong Xu
Nov 12, 2013·Journal of Dairy Science·Manuel KrewinkelLutz Fischer
Jan 7, 2014·Applied Biochemistry and Biotechnology·Vijayanand AdapaK R S Sambasiva Rao
Aug 10, 2015·Journal of Dairy Science·Eva RentschlerLutz Fischer
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Jul 2, 2016·Journal of Biomolecular Structure & Dynamics·Faez Iqbal KhanDong-Qing Wei
Sep 27, 2016·Frontiers in Microbiology·Margarita SantiagoLoreto P Parra
Nov 23, 2016·Journal of the Science of Food and Agriculture·Qiuming ChenWanmeng Mu
Mar 3, 2017·Journal of Agricultural and Food Chemistry·Beatrice KuschelLutz Fischer
Jan 24, 2018·Proceedings of the National Academy of Sciences of the United States of America·Yun-Wei DongGeorge N Somero
May 23, 2018·Nucleic Acids Research·Andrew WaterhouseTorsten Schwede
Sep 27, 2018·Nucleic Acids Research·Lorenz Christian ReimerJörg Overmann
Aug 12, 2019·International Journal of Biological Macromolecules·Yaqin XiaoWanmeng Mu

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