Computer analysis of two-dimensional electrophoresis gels: a new segmentation and modeling algorithm

Electrophoresis
E BettensP Van Osta

Abstract

The complexity of the spot patterns of two-dimensional electrophoresis gels made it necessary to use image processing techniques to analyze the gels. An important issue in the analysis is the detection and quantification of the protein spots. In this paper we describe a new technique to segment and model the different spots. For the segmentation of a gel into its different spot regions we apply a watershed technique, which is robust and efficient. For the quantification of the spots, a new spot model is constructed, based on diffusion principles. Besides the advantage of having a physical interpretation, the model is demonstrated to be superior to the commonly used Gaussian models.

References

Dec 1, 1988·Electrophoresis·D G RowlandsS McKee
Feb 15, 1988·Analytical Biochemistry·A D Olson, M J Miller
Feb 29, 1980·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D Marr, E Hildreth
Jun 1, 1981·Computers and Biomedical Research, an International Journal·P F Lemkin, L E Lipkin

❮ Previous
Next ❯

Citations

Apr 14, 2005·International Journal for Parasitology·J BarrettJ V Hamilton
Mar 19, 2008·Electrophoresis·Morten Beck Rye, Bjørn K Alsberg
Nov 13, 2008·Proteomics·Olivier Langella, Michel Zivy
Nov 4, 2010·Proteomics·Andrew W DowseyMichael J Dunn
Jan 10, 2014·IEEE Journal of Biomedical and Health Informatics·Eirini KostopoulouDimitris Maroulis
Mar 10, 2017·IEEE Transactions on Nanobioscience·Michal Marczyk
Nov 6, 2018·Journal of Magnetic Resonance Imaging : JMRI·Rhea D Chitalia, Despina Kontos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.