Computer-assisted chromatin texture characterization of Feulgen-stained nuclei in a series of 331 transitional bladder cell carcinomas

The Journal of Pathology
R van VelthovenR Kiss

Abstract

The chromatin patterns of Feulgen-stained nuclei in a series of six specimens of normal mucosa and 331 transitional bladder carcinomas, including 293 superficial (Ta and T1) and 38 invasive (T2-T4) cases, were quantitatively described by means of eight parameters relating to densitometric, run-length distribution, and co-occurrence matrix features. The results show that the chromatin texture of the superficial lesions was markedly different from that of the invasive tumours, which exhibited a distinctly more dense and heterogeneous chromatin pattern. The data also show that the increasing level of malignancy, as revealed by the increasing clinical stage, was accompanied by an increase in the overall chromatin condensation level. Only some areas of the nucleus actually increased in density; other pale areas appeared concomitantly with these increasingly denser chromatin areas. This chromatin density increase corresponded to a marked increase in the frequency of small dense chromatin clumps; these joined together into very large dense chromatin clumps, which were distributed more and more heterogeneously in the nucleus as the clinical stage of the tumour increased.

References

Jan 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G BrugalD Adelh
Jul 1, 1992·The Journal of Urology·H G van der PoelJ A Schalken
Jan 1, 1991·Cytometry·U de MeesterH C van der Linden
Apr 1, 1990·The Journal of Urology·C de PrezR Van Velthoven
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Feb 1, 1983·Human Pathology·E C OomsR W Veldhuizen
Jun 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R KissJ L Pasteels

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Citations

Jun 6, 1997·Journal of Neuroscience Methods·T W PopeM A Gordon
Sep 30, 2006·Artificial Intelligence in Medicine·Dimitris K TasoulisMichael N Vrahatis
Aug 23, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Nor F RajabValerie J McKelvey-Martin
Mar 10, 1998·Ultrastructural Pathology·K YogesanH E Danielsen

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